Characterization of a spontaneously arising murine squamous cell carcinoma (SCC VII) as a prerequisite for head and neck cancer immunotherapy
Characterization of a spontaneously arising murine squamous cell carcinoma (SCC VII) as a prerequisite for head and neck cancer immunotherapy
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DOI：
10.1002/hed.1130
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发表时间：
2001-10-01
影响因子：
2.9
通讯作者：
Strome, SE
中科院分区：
文献类型：
--
作者：
Khurana, D;Martin, EA;Strome, SE
背景。为了开发头颈部恶性肿瘤患者的新治疗方法，需要确定免疫原性差的小鼠鳞状细胞癌（SCC）模型。研究了自发性小鼠鳞状细胞癌（SCC VII）的表型、生长特征和对肿瘤特异性t细胞转移的反应。SCC VII表达主要组织相容性复合体（MHC） I类分子，但对肿瘤特异性t细胞杀伤具有抗性，对淋巴因子激活杀手（LAK）细胞介导的杀伤相对不敏感。接种5 × 10(4)个细胞后，皮内肿瘤可重复建立，静脉注射2.5 × 10(4)个细胞后，全身微转移明显。使用肿瘤特异性T细胞和全身白介素-2 （IL-2）对3天肺转移瘤进行免疫治疗在减少体内转移瘤数量方面无效。结论。SCC VII是一种免疫原性差的小鼠鳞状细胞癌，它代表了上呼吸道SCC患者免疫治疗方法临床前试验的理想模型。(C) 2001约翰威利父子公司
Background. To develop novel therapeutic approaches for patients with head and neck malignancies, poorly immunogenic murine models of squamous cell carcinoma (SCC) need to be defined.Methods. The phenotype, growth characteristics, and responsiveness to tumor-specific T-cell transfer of a spontaneously arising murine SCC (SCC VII) were characterized.Results. SCC VII expresses major histocompatibility complex (MHC) class I molecules yet is resistant to tumor-specific T-cell killing and relatively insensitive to killing mediated by lymphokine-activated killer (LAK) cells. Intradermal tumors are reproducibly established after vaccination of 5 x 10(4) cells, and systemic micrometastases are apparent after intravenous administration of 2.5 x 10(4) Cells. Immunotherapy of 3-day lung metastases using tumor-specific T cells and systemic interleukin-2 (IL-2) was ineffective in reducing the number of metastases in vivo. Conclusions. SCC VII is a poorly Immunogenic murine squamous cell cancer, which represents an ideal model for preclinical testing of immunotherapeutic, approaches for patients with SCC of the upper aerodigestive tract. (C) 2001 John Wiley & Sons, Inc.