Cadherin-6 gene regulatory patterns in the postnatal mouse brain
Cadherin-6 gene regulatory patterns in the postnatal mouse brain
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DOI：
10.1016/j.mcn.2008.05.020
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发表时间：
2008-09-01
影响因子：
3.5
通讯作者：
Inoue, Takayoshi
中科院分区：
文献类型：
--
作者：
Inoue, Yukiko U.;Asami, Junko;Inoue, Takayoshi
钙粘蛋白-6(CDH6，K-钙粘蛋白)是一种突触黏附分子，它的表达界定了出生后小鼠大脑中一系列受限的神经回路。虽然钙粘附素在突触连接的形成和/或调节中的作用已经被牵连，但驱动钙粘附素沿着大脑功能回路表达的机制仍然难以捉摸。在这里，我们通过应用一种方法来研究cdh6表达的遗传控制，该方法允许报告盒系统地整合到细菌人工染色体中，并广泛覆盖巨大的cdh6基因位点，从而在稳定的转基因小鼠系中有效地评估报告活性。这样的筛选显示，可分割的基因组片段在正确的时相差异地控制每个大脑区域或核特定的CDH6表达，以形成电路。这些可分离的钙粘附素表达调控模块倾向于根据工作连接进行分组，这表明它们在阐述大脑回路方面的发育和/或进化价值。(C)2008 Elsevier Inc.保留所有权利。
Cadherin-6 (Cdh6, K-cadherin) is a synaptic adhesion molecule the expression of which demarcates restricted sets of neuronal circuitries in postnatal mouse brains. While roles for the cadherins in the formation and/or modulation of synaptic junctions have been implicated, that which drives cadherin expression along functional brain circuits has remained elusive. Here we investigate the genetic control of Cdh6 expression by applying a method that permits systematic integration of a reporter cassette into bacterial artificial chromosomes with extensive coverage of the huge Cdh6 gene locus, whereby the reporter activities are efficiently evaluated in stable transgenic mouse lines. Such screenings revealed that divisible genomic segments differentially control each brain region or nucleus specific expression of Cdh6 at the right phases for circuit formation. These separable regulatory modules for cadherin expressions tended to be grouped by working connectivities, suggesting their developmental and/or evolutional value in elaborating brain circuitry. (C) 2008 Elsevier Inc. All rights reserved.