Involvement of CsERF2 in leaf variegation of Cymbidium sinense 'Dharma'
Involvement of CsERF2 in leaf variegation of Cymbidium sinense 'Dharma'
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CsERF2 参与大花蕙兰叶片杂色变化 – Dharma –
DOI：
10.1007/s00425-020-03426-x
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发表时间：
2020-07-28
期刊：
影响因子：
4.3
通讯作者：
Zhu, Genfa
中科院分区：
文献类型：
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作者：
Gao, Jie;Liang, Di;Zhu, Genfa
主要结论 CsERF2 是一种乙烯响应因子，在叶片杂色中发挥作用。叶片杂色是大花蕙兰（C.sinense）的主要观赏特征。然而，叶子颜色杂色的机制仍不清楚。在本研究中，我们分析了国兰'Dharma'野生型（WT）和叶片杂色突变体的叶片的细胞学和生理特征以及分子分析。在WT和突变体叶片的绿色部分(MG)中发现了具有典型和功能性结构的叶绿体，但在突变体叶片的黄色部分(MY)中没有发现。 MY中参与叶绿素（Chl）降解的关键酶的活性及其底物含量显着增加。与叶绿素降解相关的基因在 MY 中也表现出显着上调。转录组分析显示，所有已鉴定的乙烯反应因子（ERF）的表达均显着上调，并且MY中的1-氨基环丙烷-1-羧酸（ACC）含量显着高于MG。 QRT-PCR 分析证实，与叶绿素降解相关的基因的表达水平可能受到乙烯 (ETH) 处理的积极影响。 CsERF2在烟草(N. tabacum)中的稳定过表达导致叶绿体含量下降和叶绿体异常。转录组分析和qRT-PCR结果表明，与叶绿体发育和功能相关的KEGG通路在转基因N中出现显着变化。烟草。因此，茶树叶色的形成很大程度上受叶绿体发育和叶绿素代谢的影响。CsERF2在茶树叶片杂色中发挥着重要作用。
Main conclusion CsERF2, an ethylene response factor, plays a role in leaf variegation. Leaf variegation is a main ornamental characteristic inCymbidium sinense(C.sinense). However, the mechanisms of leaf color variegation remain largely unclear. In the present study, we analyzed the cytological and physiological features, as well as molecular analyses of leaves from wild-type (WT) and leaf variegation mutants ofCymbidium sinense'Dharma'. Chloroplasts with typical and functional structures were discovered in WT and green sectors of the mutants leaves (MG), but not in yellow sectors of the mutant leaves (MY). The activities of key enzymes involved in chlorophyll (Chl) degradation and their substrate contents were significantly increased in MY. Genes related to Chl degradation also showed a significant up-regulation in MY. Transcriptomic analysis showed that the expression of all identified ethylene response factors (ERFs) was significantly up-regulated, and the 1-aminocyclopropane-1-carboxylic acid (ACC) content in MY was significantly higher compared with MG. QRT-PCR analysis validated that the expression levels of genes related to Chl degradation could be positively affected by ethylene (ETH) treatment. Stable overexpression ofCsERF2inNicotiana tabacum(N. tabacum) led to a decrease in Chl content and abnormal chloroplast. Transcriptomic analysis and qRT-PCR results showed that the KEGG pathway related to chloroplast development and function showed significant change in transgenicN. tabacum. Therefore, the leaf color formation ofC.sinensewas greatly affected by chloroplast development and Chl metabolism.CsERF2played an important role in leaf variegation ofC.sinense.