Effects of metallic debris on adult bovine articular chondrocyte metabolism in vitro
Effects of metallic debris on adult bovine articular chondrocyte metabolism in vitro
复制标题
金属碎片对成年牛关节软骨细胞体外代谢的影响
DOI：
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
R. L. Smith
中科院分区：
文献类型：
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作者：
W. Maloney;F. Castro;D. Schurman;R. L. Smith
本研究的目的是检查来自关节假体中常用金属的颗粒对体外软骨细胞增殖、代谢和形态的影响。软骨细胞活力受所添加的金属颗粒的类型和浓度的影响。钴在所有颗粒浓度（0.83- 0.000083%，v/v）下对软骨细胞都是有毒的，而铬、钛和钛-铝颗粒仅在高浓度下影响软骨细胞活力。通过酪蛋白酶、胶原酶和氨基己糖苷酶活性评估，软骨细胞对颗粒碎片的代谢反应在低浓度下是可变的，但在高浓度（0.83% v/v）下总是降低。前列腺素E2水平在培养基中显示出稳定的增加时，颗粒负荷增加，除了在培养基中的软骨细胞暴露于钛铝。软骨细胞暴露于钛的扫描电子显微镜显示皱褶的细胞边界和频繁的膜起泡。这与暴露于钴的软骨细胞形成对比，其中锯齿状外观表明细胞死亡，而钛-铝则显示细胞静止。这些研究结果表明，金属颗粒改变软骨细胞的活力和代谢，并表明颗粒碎片可能会影响半关节置换术后关节软骨的完整性和稳定性。John Wiley & Sons，Inc.
The purpose of this study was to examine the effects of particles, derived from metals commonly used in joint prostheses, on chondrocyte proliferation, metabolism, and morphology in vitro. Chondrocyte viability was influenced by the type and concentration of metal particle added. Cobalt was toxic to chondrocytes at all particle concentrations (0.83–0.000083%, v/v), whereas the chromium, titanium and titanium-aluminum particles only effected chondrocyte viability at high concentrations. The metabolic response of chondrocytes to particulate debris as assessed by caseinase, collagenase, and hexosaminidase activities were variable at low concentrations but were always reduced at high concentrations (0.83% v/v). Prostaglandin E2 levels in the medium showed a steady increase when particle load increased, except in the medium of chondrocytes exposed to titanium-aluminum. Scanning electron microscopy of chondrocytes exposed to titanium showed ruffled cell borders and frequent membrane blebbings. This was in contrast to chondrocytes exposed to cobalt, where the crenated appearance indicated cell death, and titanium-aluminum, where the cells appeared quiescent. These findings show that metal particles alter chondrocyte viability and metabolism and suggest that particulate debris may influence the integrity and stability of articular cartilage following hemiarthroplasty. © 1994 John Wiley & Sons, Inc.
使用可溶性放射性胶原底物进行简单的脊椎动物胶原酶测定。
影响因子：
2.9
作者：
Seng,WL;Davison,PF;Kenyon,KR
通讯作者：
Kenyon,KR