Gut Microbiota Reconstruction Following Host Infection with Blood-stage Plasmodium berghei ANKA Strain in a Murine Model
Gut Microbiota Reconstruction Following Host Infection with Blood-stage Plasmodium berghei ANKA Strain in a Murine Model
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DOI：
10.1007/s11596-019-2119-y
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发表时间：
2019-12-01
影响因子：
2.4
通讯作者：
Fang, Min
中科院分区：
文献类型：
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作者：
Fan, Zhi-gang;Li, Xiao;Fang, Min
疟疾仍然是一个全球性的健康问题。恶性疟原虫与新月疟原虫的关系。以及肠道微生物区系以及疟原虫的影响。脊椎动物宿主肠道微生物区系的研究尚不清楚。本研究的目的是评价血液期疟原虫对小鼠肠道微生物区系的影响。通过16S rRNA测序和生物信息学分析，分三个阶段对肠道微生物区系进行分析。肠道微生物区系在健康阶段、感染阶段和治愈阶段(消灭疟疾后第9天)发生了变化。此外，这些受感染动物的肠道微生物区系在感染疟疾后没有恢复。在所有三个阶段都有254个可操作的分类单位(OTU)，在实验的每个阶段都有独特的菌株或OTU。8个OTUS的群落丰富度百分比变化显著(P
Malaria remains a global health problem. The relationship between Plasmodium spp. and the gut microbiota as well as the impact of Plasmodium spp. on the gut microbiota in vertebrate hosts is unclear. The aim of the current study was to evaluate the effect of blood-stage Plasmodium parasites on the gut microbiota of mice. The gut microbiota was analyzed by 16S rRNA sequencing and bioinformatic analyses at three stages. The gut microbiota changed during the three phases: the healthy stage, the infection stage, and the cure stage (on the 9th day after malarial elimination). Moreover, the gut microbiota of these infected animals did not recover after malaria infection. There were 254 operational taxonomic units (OTUs) across all three stages, and there were unique strains or OTUs at each stage of the experiment. The percentages of community abundance of 8 OTUs changed significantly (P