Expression of calbindin-D28k in developing and growing chick testes.
Expression of calbindin-D28k in developing and growing chick testes.
复制标题
钙结合蛋白-D28k 在发育和生长的小鸡睾丸中的表达。
DOI：
10.1007/bf00270035
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Taylor,AN
中科院分区：
文献类型：
--
作者：
Inpanbutr,N;Taylor,AN
钙结合蛋白 (Calbindin) 是一种 28 kDa 维生素 D 依赖性钙结合蛋白，通过免疫组织化学方法定位于发育和生长的小鸡睾丸中。该蛋白质首先出现在八天大胚胎的发育睾丸的生发上皮中，并在整个发育过程中保留在其中。孵化后的生发上皮中不存在钙结合蛋白。接下来在一周龄和正在发育的小鸡睾丸的精原细胞和精母细胞中检测到钙结合蛋白。随着生精小管的进一步发育，钙结合蛋白阳性精原细胞和精母细胞的数量和染色强度逐渐增加。五周龄及以上雏鸡的一些间质间质细胞钙结合蛋白呈阳性。比较鸡精原细胞和精母细胞中钙结合蛋白含量的出现和增加与精子发生的时间过程，表明钙结合蛋白可能参与精原细胞和精母细胞的有丝分裂过程。
Calbindin, a 28-kDa vitamin D-dependent calcium-binding protein was localized immunohistochemically in developing and growing chick testes. The protein first appeared in the germinal epithelium of developing testes of the eight-day-old embryo and remained therein throughout development. Calbindin was not present in the germinal epithelium after hatching. Calbindin was next detected in the spermatogonia and spermatocytes of one-week-old and growing chick testes. Calbindin-positive spermatogonia and spermatocytes gradually increased in number and staining intensity as the seminiferous tubules further developed. A few interstitial Leydig cells were positive for calbindin from five-week-old and older chicks. Comparison of the time-course of appearance and increase in calbindin content in spermatogonia and spermatocytes with spermatogenesis in chickens suggests that calbindin may be involved in the mitotic process in spermatogonia and spermatocytes.