Tissue reaction to urogynecologicmeshes: effect of steroid soaking in two different mesh models
Tissue reaction to urogynecologicmeshes: effect of steroid soaking in two different mesh models
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DOI：
10.1007/s00192-016-3013-9
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发表时间：
2016-10-01
影响因子：
1.8
通讯作者：
Sahin, Barbaros
中科院分区：
文献类型：
--
作者：
Karabulut, Aysun;Simavli, Serap Aynur;Sahin, Barbaros
介绍和假设类固醇浸泡可以减少组织中网状物引发的炎症反应。我们的目的是调查的组织反应，以类固醇浸泡的网状材料和未浸泡的网状材料在大鼠model.Methods中性和类固醇浸泡的I型大孔聚丙烯（PP）单丝和聚偏氟乙烯（PVF）网状材料植入腹直肌的20个成熟的Wistar白化病大鼠。将动物分为四组：含类固醇的PP补片（PP-S）、不含类固醇的PP补片、含类固醇的PVF补片（PVF-S）和不含类固醇的PVF补片。12周后处死大鼠，进行组织学、免疫组化和电镜检查。对于免疫组织化学分析，多克隆兔抗小鼠CD 3、兔抗小鼠CD 68、兔抗小鼠CD 15和兔抗小鼠CD 34抗体分别用于检测淋巴细胞、巨噬细胞、多形性白细胞异物巨细胞和纤维肌细胞干细胞。用苏木精和伊红对样本进行染色，用于炎症的组织学评价，用Masson三色染色法对胶原沉积进行评价。结果PVF、PVF-S和PP-S组的CD 3表达低于PP组，而PVF-S和PP-S组的CD 34表达高于PP组（p0.05）。
Introduction and Hypothesis Steroid soaking may decrease mesh-triggered inflammatory reaction in tissue. We aimed to investigate the tissue reaction to a steroid-soaked mesh material and an unsoaked mesh material in the rat model.Methods Neutral and steroid-soaked type I macroporous polypropylene (PP) monofilament and polyvinylidene fluoride (PVF) mesh materials were implanted on the rectus abdominis muscle of 20 mature Wistar albino rats. Animals were divided into four groups: PP mesh with steroid (PP-S), PP mesh without steroid, PVF mesh with steroid (PVF-S), and PVF mesh without steroid. The rats were killed after 12 weeks, and histologic, immunohistochemical and electron microscopic examinations were performed. For immunohistochemical analysis, polyclonal rabbit anti-mouse CD3, rabbit anti mouse CD68, rabbit anti-mouse CD15, and rabbit anti-mouse CD34 antibodies were used for the detection of lymphocytes, macrophages, polymorphonuclear leukocyte foreign body giant cells, and fibromyocyte stem cells, respectively. Samples were stained with hematoxylin and eosin for the histologic evaluation of inflammation and with Masson's trichrome stain for the evaluation of collagen deposition. Pore size and mesh ultrastructure were evaluated by electron microscopy.Results Expression of CD3 was lower in the PVF, PVF-S and PP-S groups, and expression of CD34 was higher in the PVF-S and PP-S groups than in the PP groups (p 0.05).Conclusions PVF mesh induces less inflammation than PP mesh, and in both mesh types steroid soaking further decreases inflammation without changing the pore size.