The N-Acetylmannosamine Transferase Catalyzes the First Committed Step of Teichoic Acid Assembly in Bacillus subtilis and Staphylococcus aureus
The N-Acetylmannosamine Transferase Catalyzes the First Committed Step of Teichoic Acid Assembly in Bacillus subtilis and Staphylococcus aureus
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DOI：
10.1128/jb.00611-08
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发表时间：
2009-06-15
影响因子：
3.2
通讯作者：
Brown, Eric D.
中科院分区：
文献类型：
--
作者：
D'Elia, Michael A.;Henderson, James A.;Brown, Eric D.
近年来，在磷壁酸生物合成基因的可有可有性的表征方面取得了相当大的进展。到目前为止，一个值得注意的遗漏是磷壁酸合成中编码N-乙酰甘露糖胺转移酶(枯草芽孢杆菌中的TagA；金黄色葡萄球菌中的Tara)的早期基因，该基因添加N-乙酰甘露糖胺以完成十一碳烯醇焦磷酸连接的二糖的合成。在这里，我们证明了N-乙酰甘露糖胺转移酶对体外生长是必不可少的，使这种生物合成酶成为该途径中最后一个可有可无的基因，这表明TagA(或Tara)编码了壁磷壁酸合成的第一步。
There have been considerable strides made in the characterization of the dispensability of teichoic acid biosynthesis genes in recent years. A notable omission thus far has been an early gene in teichoic acid synthesis encoding the N-acetylmannosamine transferase (tagA in Bacillus subtilis; tarA in Staphylococcus aureus), which adds N-acetylmannosamine to complete the synthesis of undecaprenol pyrophosphate-linked disaccharide. Here, we show that the N-acetylmannosamine transferases are dispensable for growth in vitro, making this biosynthetic enzyme the last dispensable gene in the pathway, suggesting that tagA (or tarA) encodes the first committed step in wall teichoic acid synthesis.