Comparative transcriptomic analysis of Gardnerella vaginalis biofilms vs. planktonic cultures using RNA-seq.
Comparative transcriptomic analysis of Gardnerella vaginalis biofilms vs. planktonic cultures using RNA-seq.
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DOI：
10.1038/s41522-017-0012-7
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发表时间：
2017
影响因子：
9.2
通讯作者：
Cerca N
中科院分区：
文献类型：
--
作者：
Castro J;França A;Bradwell KR;Serrano MG;Jefferson KK;Cerca N
细菌性阴道病是影响育龄妇女最常见的妇科疾病。细菌性阴道病通常与阴道加德纳菌生物膜的发展有关。最近的数据表明G.阴道生物膜对抗生素的耐受性更强，并且能够掺入其它细菌性阴道病相关物种，从而产生多物种生物膜。然而，尽管其在细菌性阴道病中的作用明显，但对G.流浪汉为了深入了解G.为了研究细菌性阴道病发病机制中的生物膜蛋白质表达，我们采用RNA测序法对生物膜蛋白质表型和生物膜蛋白质表型进行了比较转录组学分析。815个基因的表达水平存在显著差异。基于直接和功能性基因相互作用对所获得的结果进行了详细分析。与其他细菌物种相似，生物膜细胞中参与抗菌素耐药性的基因表达升高。此外，我们的数据表明，G.迷走神经生物膜呈现对应激和饥饿条件的特征性反应。生物膜中编码参与葡萄糖和碳代谢的蛋白质的转录物的丰度降低。令人惊讶的是，阴道溶素的转录水平在生物膜中相对于无菌培养物降低。总体而言，我们的数据表明，基因调控的过程中，G。阴道生物膜导致细菌生长受到保护，其特征是代谢活性低。这种表型可能有助于细菌性阴道病的慢性和复发性。这表明G.迷走神经能够通过基因表达的广泛变化来显著调节其表型。改变的基因活性可能会促进育龄妇女最常见的妇科疾病的慢性和复发性感染。细菌性阴道病（BV）经常涉及由阴道加德纳菌属细菌形成的生物膜。葡萄牙米尼奥大学的Nuno Cerca和同事，以及葡萄牙其他地方和美国的同事，研究了BV生物膜中基因活性的变化。他们的结果将815个基因的活性差异与细菌在产生生物膜时进入受保护的生长形式的能力联系起来。这些差异包括与抗生素耐药性相关的特定基因的活性增加。这种对细菌基因活性发生剧烈变化的能力的洞察可以指导理解慢性和复发性BV的工作。它也可能导致新的方法来治疗这种常见和麻烦的情况。
Bacterial vaginosis is the most common gynecological disorder affecting women of reproductive age. Bacterial vaginosis is frequently associated with the development of a Gardnerella vaginalis biofilm. Recent data indicates that G. vaginalis biofilms are more tolerant to antibiotics and are able to incorporate other bacterial vaginosis -associated species, yielding a multi-species biofilm. However, despite its apparent role in bacterial vaginosis, little is known regarding the molecular determinants involved in biofilm formation by G. vaginalis. To gain insight into the role of G. vaginalis in the pathogenesis of bacterial vaginosis, we carried out comparative transcriptomic analysis between planktonic and biofilm phenotypes, using RNA-sequencing. Significant differences were found in the expression levels of 815 genes. A detailed analysis of the results obtained was performed based on direct and functional gene interactions. Similar to other bacterial species, expression of genes involved in antimicrobial resistance were elevated in biofilm cells. In addition, our data indicate that G. vaginalis biofilms assume a characteristic response to stress and starvation conditions. The abundance of transcripts encoding proteins involved in glucose and carbon metabolism was reduced in biofilms. Surprisingly, transcript levels of vaginolysin were reduced in biofilms relative to planktonic cultures. Overall, our data revealed that gene-regulated processes in G. vaginalis biofilms resulted in a protected form of bacterial growth, characterized by low metabolic activity. This phenotype may contribute towards the chronic and recurrent nature of bacterial vaginosis. This suggests that G. vaginalis is capable of drastically adjusting its phenotype through an extensive change of gene expression. Altered gene activity may promote chronic and recurrent infections in the most common gynecological disorder in women of reproductive age. Bacterial vaginosis (BV) frequently involves the formation of biofilms by Gardnerella vaginalis bacteria. Nuno Cerca and colleagues at the University of Minho in Portugal, with co-workers elsewhere in Portugal and in the USA, studied changes in gene activity in BV biofilms. Their results linked differences in the activity of 815 genes to an ability of the bacteria to enter a protected form of growth when producing biofilms. The differences included increased activity of specific genes associated with antibiotic resistance. This insight into the bacterial ability to undergo drastic changes in gene activity could guide work to understand chronic and recurrent BV. It may also lead to new ways to treat this common and troublesome condition.