Clearing skeletal muscle with CLARITY for light microscopy imaging.
Clearing skeletal muscle with CLARITY for light microscopy imaging.
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DOI：
10.1002/cbin.10578
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发表时间：
2016-04
影响因子：
3.9
通讯作者：
Ralston E
中科院分区：
文献类型：
--
作者：
Milgroom A;Ralston E
在大的组织体积上观察亚细胞细节正在成为旨在可视化整个器官或组织中细胞连接的大规模项目成功的必要条件。然而，组织不透明性仍然是深层组织成像的障碍。这种情况带来了组织清除技术的新的兴趣;最近开发了新的方案，如CLEAITY（透明脂质交换丙烯酰胺杂交刚性成像/免疫染色/原位杂交相容组织-水凝胶）。到目前为止，这些技术的大多数测试已经应用于大脑或其他软组织。在这里，我们表明，EQUITY清除小鼠后肢骨骼肌，并保持肌肉及其纤维的基本结构特征。然而，用荧光标记物标记并不成功。
Viewing subcellular details over large tissue volumes is becoming an essential condition of the success of large-scale projects aimed at visualizing cell connections in whole organs or tissues. However, tissue opacity remains an obstacle to deep tissue imaging. This situation has brought renewed interest for techniques of tissue clearing; new protocols, such as CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/In situ hybridization-compatible Tissue-hYdrogel), have recently been developed. So far, most of the tests of these techniques have been applied to brain or other soft tissues. Here we show that CLARITY clears mouse hindlimb skeletal muscles and maintains the basic structural features of muscle and its fibers. However, tagging with fluorescent markers was not successful.