EFFECT OF CYCLOPHOSPHAMIDE ON LEUKOCYTIC SUBSET DISTRIBUTIONS IN RATS CARRYING THE DUNNING R3327-MAT-LYLU PROSTATIC ADENOCARCINOMA
EFFECT OF CYCLOPHOSPHAMIDE ON LEUKOCYTIC SUBSET DISTRIBUTIONS IN RATS CARRYING THE DUNNING R3327-MAT-LYLU PROSTATIC ADENOCARCINOMA
复制标题
DOI：
10.1002/pros.2990110203
复制
发表时间：
1987-01-01
期刊：
影响因子：
2.8
通讯作者：
GUINAN, PD
中科院分区：
文献类型：
--
作者：
SHAW, MW;RUBENSTEIN, M;GUINAN, PD
Dunning R3327腺癌代表了一个研究大鼠前列腺癌的模型；早期的研究表明，它对于研究肿瘤生长、免疫标记物和化疗之间的关系是有用的。用10、30和100 mg/kg剂量的环磷酰胺(CTX)处理正常动物和转移性黑色素瘤R3327 Mat-LyLu的小鼠，用单抗检测小鼠脾中白细胞亚群的分布。与对照组相比，携带肿瘤的动物的辅助T细胞含量显著减少，辅助/抑制T细胞比率也降低。这些效应在植入后迅速发生，且不能被化疗逆转。当CTX同时用于荷瘤和非荷瘤动物时，也会耗尽T细胞群。尽管在所有亚群中都产生了减少，但两次注射CTX(30毫克/公斤)能够保持(在非荷瘤动物中)或恢复(在荷瘤动物中)正常的辅助/抑制T细胞比率。这类研究有助于确定细胞毒药物的治疗有效剂量，将其对免疫系统的有害影响降至最低。
The Dunning R3327 adenocarcinoma represents a model for studying prostate cancer in rats; early studies have indicated its utility for studying relationship between tumor growth, immunologic markers, and chemotherapy. Normal animals and those bearing the metastatic Dunning R3327 MAT-LyLu tumor were treated with 10, 30, and 100 mg/kg doses of cyclophosphamide (CTX) and their spleens assayed for leukocytic subset distributions using monoclonal antibodies. Tumor-bearing animals had significant reductions in helper T cell content as well as reduced helper/suppressor T cell ratios, compared to controls. These effects occurred rapidly following implantation and were not reversed by chemotherapy. When administered to both tumor- and non-tumor-bearing animals, CTX also depleted T cell populations. Despite reductions produced in all subsets, two administrations of CTX (30 mg/kg) were capable of retaining (in non-tumor-bearing animals) or restoring (in tumor-bearing) normal helper/suppressor T cell ratios. Such studies aid in identifying therapeutically effective dosages of cytotoxic drugs that minimize their deleterious effects on the immune system.