CORRELATION BETWEEN CELL-CYCLE DURATION AND RNA-CONTENT
CORRELATION BETWEEN CELL-CYCLE DURATION AND RNA-CONTENT
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DOI：
10.1002/jcp.1041000306
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发表时间：
1979-01-01
影响因子：
5.6
通讯作者：
MELAMED, ML
中科院分区：
文献类型：
--
作者：
DARZYNKIEWICZ, Z;EVENSON, DP;MELAMED, ML
异染荧光染料吖啶橙子被用来在中国仓鼠卵巢（CHO）细胞和有丝分裂原刺激的人淋巴细胞在其通过细胞周期的进展过程中的DNA和RNA的差异染色。通过流式细胞术测量分别代表细胞DNA和RNA的单个细胞的绿色和红色荧光。通过在有丝分裂时选择性分离使CHO细胞同步化。它们通过G1期和随后通过S期的进展速率与mRNA的含量相关。G1期的平均持续时间为5.2小时的细胞与高RNA含量（最高的25百分位数的人口）和8.1小时的细胞与低RNA（最低的25百分位数）。S期的持续时间为5.9和7.5小时的高和低RNA，25百分位数的亚群，分别。同步化的淋巴细胞在G1/S边界的羟基脲或5-氟脱氧尿苷表现出极高的细胞间变异相对于mRNA的含量。从封闭物中释放后，它们以与mRNA含量成线性比例的速率穿过S相。高RNA含量细胞的S期持续时间为5 h，中等RNA含量细胞的S期持续时间为6-9 h，最低RNA含量细胞的S期持续时间为27 h。群体内单个细胞的细胞周期进展速率可能与每个细胞的核糖体数量相关。
The metachromatic fluorochrome acridine orange was used to differentially stain DNA and RNA in Chinese hamster ovary (CHO) cells and in mitogen-stimulated human lymphocytes during their progression through the cell cycle. Green and red fluorescence of individual cells, representing cellular DNA and RNA, respectively, was measured by flow cytometry. CHO cells were synchronized by selective detachment at mitosis. Their rate of progression through G1 and subsequently through S phase correlated with the content of stainable RNA. The mean duration of the G1 phase was 5.2 h for cells with high RNA content (highest 25 percentile population) and 8.1 h for cells with low RNA (lowest 25 percentile). The duration of S phase was 5.9 and 7.5 h for high- and low-RNA, 25 percentile subpopulations, respectively. Lymphocytes synchronized at the G1/S boundary by hydroxyurea or 5-fluorodeoxyuridine showed extremely high intercellular variation with respect to content of stainable RNA. After release from the block they traversed S phase at rates linearly proportional to the content of stainable RNA. The duration of S phase was 5 h for cells with high RNA-, 6-9 h for cells with moderate RNA- and up to 27 h for cells with minimal RNA-content. The rate of progression through the cell cycle of individual cells within a population may be correlated with the number of ribosomes per cell.