Ontogenic expression of putative feeding peptides in the rat fetal brain and placenta.
Ontogenic expression of putative feeding peptides in the rat fetal brain and placenta.
复制标题
大鼠胎儿脑和胎盘中推定喂养肽的个体表达。
DOI：
10.1080/10284150600630676
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Ross,MG
中科院分区：
文献类型：
--
作者：
Beloosesky,R;Gayle,DA;Amidi,F;Ahanya,SN;Desai,M;Ross,MG
经过充分证明的“胎儿编程”范例是基于这样的观察，即环境变化可以重置发育路径，从而重置子宫内发育过程中的基因表达。随着食欲调节神经通路在子宫内的发育，我们试图确定在妊娠最后三分之一的胎鼠大脑和胎盘中可能存在的食欲性和厌食性摄食调节多肽的个体表达。取孕14、16、18天的SD大鼠12只，取胎儿全脑和胎盘，用实时定量逆转录聚合酶链式反应(RT-PCR)检测食欲素(神经肽Y、刺鼠相关肽(AgRP))、厌食症(可卡因和苯丙胺调节转录物(CART)、阿片黑素皮质素原(POMC))多肽和瘦素受体(OB-RB)的mRNA水平。为了与成年大鼠进行比较，研究人员还检查了雄性大鼠的下丘脑、皮质和小脑的摄取肽。在胎脑和胎盘中，AgRP的mRNA水平从14天到16天下降了10倍，在18天时检测不到。食欲抑制因子OB-Rb和CART在脑和胎盘中的表达水平从第14天到第18天逐渐升高。从第14天到第18天，NPY和POMC的表达没有变化。摄食调节肽在胎儿大脑中的表达模式与成人大脑皮层的表达模式最为相似。妊娠晚期喂养调节机制的持续成熟表明了在子宫内对摄食行为进行编程的可能性。
The well-demonstrated “fetal programming” paradigm is based on the observation that environmental changes can reset the developmental path and thus, gene expression during intrauterine development. As appetite-regulatory neural pathways develop in utero, we sought to determine the ontogenic expression of putative orexigenic and anorexigenic feeding-regulatory peptides in the fetal rat brain and placenta during the last third of gestation. Pregnant Sprague-Dawley rats ( n = 12) at D14, D16 and D18 were sacrificed and fetal whole brain and placenta removed and examined for mRNA levels of orexigenic (neuropeptide Y (NPY), agouti-related peptide (AgRP)) and anorexigenic (cocaine and amphetamine regulated transcript (CART), pro-opiomelanocortin (POMC)) peptides and leptin receptor (OB-Rb) using real-time reverse transcription polymerase chain reactions (RT-PCR). For adult comparisons, the hypothalamus, cortex and cerebellum from male rats were also examined for feeding peptides. In the fetal brain and placenta, mRNA levels of AgRP decreased 10-fold from D14 to D16 and was undetectable at D18. Appetite inhibitory factors OB-Rb and CART mRNA levels increased from D14 to D18 in the brain and placenta. NPY and POMC expression remained unchanged from D14 to D18. The pattern of expression of feeding regulatory peptides in the fetal brain most closely resembled the expression profile of the adult cerebral cortex. The continued maturation of feeding regulatory mechanisms in late gestation indicates the potential for in utero programming of ingestive behavior.