Protein detection by optical shift of a resonant microcavity
Protein detection by optical shift of a resonant microcavity
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DOI：
10.1063/1.1482797
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发表时间：
2002-05-27
影响因子：
4
通讯作者：
Arnold, S
中科院分区：
文献类型：
--
作者：
Vollmer, F;Braun, D;Arnold, S
我们提出了一种光学生物传感器与前所未有的灵敏度检测未标记的分子。我们的设备使用电介质微粒中的光学共振（回音壁模式）作为物理换能机制。通过与腐蚀光纤的倏逝波耦合来激发共振，并将其检测为在不同波长下通过光纤传输的光强度的下降。蛋白质在微粒表面上的结合由共振波长的偏移来测量。我们证明了我们的设备的灵敏度，通过测量牛血清白蛋白的吸附，我们显示其作为生物传感器的使用，通过检测链霉亲和素结合生物素。(C)2002年美国物理学会。
We present an optical biosensor with unprecedented sensitivity for detection of unlabeled molecules. Our device uses optical resonances in a dielectric microparticle (whispering gallery modes) as the physical transducing mechanism. The resonances are excited by evanescent coupling to an eroded optical fiber and detected as dips in the light intensity transmitted through the fiber at different wavelengths. Binding of proteins on the microparticle surface is measured from a shift in resonance wavelength. We demonstrate the sensitivity of our device by measuring adsorption of bovine serum albumin and we show its use as a biosensor by detecting streptavidin binding to biotin. (C) 2002 American Institute of Physics.