Development of a new heat tolerance assay system for rice spikelet sterility.
Development of a new heat tolerance assay system for rice spikelet sterility.
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DOI：
10.1186/s13007-017-0185-3
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发表时间：
2017
期刊：
影响因子：
5.1
通讯作者：
Morita S
中科院分区：
文献类型：
--
作者：
Hakata M;Wada H;Masumoto-Kubo C;Tanaka R;Sato H;Morita S
高温诱导的小穗不育引起的水稻减产一直是水稻生产中的一个严重问题。迄今为止，已经使用了几种筛选方法，尽管它们的重现性有时很差，主要是由于品种在抽穗日期和穗高（即与灯的距离）方面的差异造成的伪影。我们开发了一种新的热致小穗不育试验系统，通过在植物园内的人工稻田进行一年的高重复性试验。对限于主茎的植株进行了一系列的高温处理，并采用高度可调盆栽保证了植株的高度均匀性。结果表明，35°c -昼/29°c -夜循环3 d热处理是最适宜的条件。在此处理下，9个耐热品种中鉴定出两个不同的群体，穗温不存在品种差异，说明该体系能够消除穗温的品种差异。结果表明，该系统可作为耐热品种选育和遗传因子分析的有力工具，消除潜在的人为因素。本文的在线版本（doi:10.1186/s13007-017-0185-3）包含补充材料，仅供授权用户使用。
Reduction in rice yield caused by high temperature-induced spikelet sterility has been a serious concern in rice production. To date, several screening methods have been used, although their reproducibility is sometimes poor due to artifacts mainly caused by varietal differences in heading dates and panicle heights (i.e., the distance from the lamps). We have developed a novel assay system for heat-induced spikelet sterility by using artificial rice paddies in phytotrons to conduct a highly reproducible assay throughout a year. Plants restricted to the main culm were treated under a series of heat conditions, and height uniformity of each plant was ensured by using height-adjustable pots. Results suggested that a 3-day heat treatment of 35 °C-day/29 °C-night cycles was the most suitable condition. Under the treatment, two distinct groups were identified among nine heat tolerant cultivars, with no varietal difference in panicle temperature, indicating that the system is capable of eliminating the varietal difference in panicle temperature. It is concluded that the assay system would be a powerful tool for selecting heat tolerant varieties, as well as the analysis of genetic factors from various cultivars, eliminating potential artifacts. The online version of this article (doi:10.1186/s13007-017-0185-3) contains supplementary material, which is available to authorized users.