COLICINOGENY OF O157-H7 ENTEROHEMORRHAGIC ESCHERICHIA-COLI AND THE SHIELDING OF COLICIN AND PHAGE RECEPTORS BY THEIR O-ANTIGENIC SIDE-CHAINS
COLICINOGENY OF O157-H7 ENTEROHEMORRHAGIC ESCHERICHIA-COLI AND THE SHIELDING OF COLICIN AND PHAGE RECEPTORS BY THEIR O-ANTIGENIC SIDE-CHAINS
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DOI：
10.1139/m91-014
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发表时间：
1991-02-01
影响因子：
2.8
通讯作者：
LIOR, H
中科院分区：
文献类型：
--
作者：
BRADLEY, DE;HOWARD, SP;LIOR, H
对来自不同临床状况患者的 20 株 O157:H7 肠出血性大肠杆菌菌株进行了大肠杆菌素生成和 Verotoxin (VT) 基因的检测。血性腹泻病例中，7/8 的分离株和溶血性尿毒症综合征病例中的 3/5 分离株均合成了似乎是大肠菌素 D 的物质。其余菌株（其中 7 株来自无症状来源）均不产生大肠杆菌素。发现确定大肠杆菌素的质粒比 5.2 kb pColD 大 1.4 kb。大肠杆菌素 D 蛋白的分子量约为 90 000，而 O157 大肠杆菌素的分子量为 87 000。该质粒被命名为 pColD157 以反映这些差异。 O157:H7 分离株中，17/20 具有 Verotoxins VT1 和 VT2 基因，其余 3/20 仅具有 VT1 基因。 VT 决定因素的存在与大肠杆菌原或症状之间没有相关性。 O157:H7 菌株对其他大肠杆菌素和噬菌体表现出显着的耐药性。
Twenty O157:H7 enterohemorrhagic Escherichia coli strains from patients with different clinical conditions were tested for colicinogeny and the presence of Verotoxin (VT) genes. From bloody diarrhea cases, 7/8 isolates and from hemolytic uremic syndrome cases 3/5 isolates all synthesized what appeared to be colicin D. The remaining strains, which included 7 from asymptomatic sources, were noncolicinogenic. The plasmid determining the colicin was found to be 1.4 kb larger than the 5.2-kb pColD. The colicin D protein had a molecular weight of about 90 000, whereas the O157 colicin was 87 000. The plasmid was designated pColD157 to reflect these differences. Of O157:H7 isolates 17/20 had genes for both of the Verotoxins VT1 and VT2, and the remaining 3/20 for VT1 only. There was no correlation between the presence of VT determinants and colicinogeny or symptoms. The O157:H7 strains exhibited significant resistance to other colicins and bacteriophages.