EFFECTS OF EXPERIMENTAL PSYCHOLOGICAL STRESS ON DISTRIBUTION AND FUNCTION OF PERIPHERAL-BLOOD CELLS
EFFECTS OF EXPERIMENTAL PSYCHOLOGICAL STRESS ON DISTRIBUTION AND FUNCTION OF PERIPHERAL-BLOOD CELLS
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DOI：
10.1097/00006842-199207000-00002
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发表时间：
1992-07-01
影响因子：
3.3
通讯作者：
BALLIEUX, RE
中科院分区：
文献类型：
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作者：
BROSSCHOT, JF;BENSCHOP, RJ;BALLIEUX, RE
50名男性受试者(年龄在24岁到55岁之间)受到轻微的、可能无法控制的人际压力环境的影响。他们被要求解一道难题。随后，他们被要求向研究人员解释他们对“另一个主题”的解决方案，实际上是一个邦联成员。邦联挫败了受试者的解释努力。人们小心翼翼地注意到，这个谜题既没有解决，也没有解释成功。与36名男性受试者的对照组相比，实验组的情绪变化证明，实验情况引起了轻微的心理紧张。分别在应激前、应激后即刻、应激后15分钟、应激30分钟后留置导尿管抽取外周血。检测白细胞、淋巴细胞、单核细胞、T细胞亚群、自然杀伤(NK)细胞和B细胞的数量。作为功能分析，我们使用了T和B细胞对有丝分裂刺激(PHA和PWM)和对抗原鸡尾酒的体外增殖反应。分析中考虑了健康和生物行为变量的潜在影响，以及初始情绪或免疫基线的附带差异。研究结果在先前研究的基础上进行了重复和扩展。与对照组相比，实验对象的NK细胞数量在应激期后显著增加，在休息15分钟后恢复到基线水平。在T-抑制/细胞毒细胞和T-辅助/抑制比上也表现出类似的作用，但这些作用可能归因于CD8+CD57+细胞数量的变化。未观察到对细胞增殖的影响。结果表明，持续时间较短的轻度心理应激源的作用主要局限于NK细胞群。
Fifty male subjects (aged 24 to 55 years) were subjected to a mild and potentially uncontrollable interpersonal stress situation. They were asked to solve a difficult puzzle. Subsequently they were requested to explain their solution to "another subject," actually a confederate to the researchers. The confederate frustrated the subjects' explanation efforts. Care was taken that neither solving nor explaining of the puzzle was successful. The experimental situation induced mild psychological strain as documented by mood changes in the experimental group when compared with a control group of 36 male subjects. Peripheral blood was drawn by an indwelling catheter just before, directly after, 15 minutes after, and 30 minutes after the stress situation. Numbers of leukocytes, lymphocytes, monocytes, T-cell subsets, natural killer (NK) cells, and B-cells were determined. As functional assays we used in vitro proliferative responses of T- and B-cells to mitogenic stimulation (PHA and PWM) and to an antigen cocktail. The potential influences of health- and biobehavioral variables were taken into account in the analyses, as well as incidental differences in initial mood or immunological baseline. The results replicated and expanded on previous research. In contrast to controls, experimental subjects showed a significant increase in numbers of NK cells after the stress-period, returning to baseline values after 15 minutes of rest. A similar effect was shown on T-suppressor/cytotoxic cells and, inversely, on T-helper/suppressor ratio, but these effects could be attributed to changes in the numbers of CD8+CD57+ cells. No effects were observed on proliferation. From the results we conclude that the effects of a short lasting mild psychological stressor are mainly restricted to cells of the NK cell population.