Satellite cells express distinct patterns of myogenic proteins in immature skeletal muscle
Satellite cells express distinct patterns of myogenic proteins in immature skeletal muscle
复制标题
卫星细胞在未成熟骨骼肌中表达不同的生肌蛋白模式
作者：
E. Schultz;C. Chamberlain;K. McCormick;P. Mozdziak
卫星细胞是位于肌纤维肌膜和基底层之间的生肌细胞。本研究的目的是确定生长中的大鼠比目鱼肌和趾长伸肌 (EDL) 肌肉中卫星细胞群中 MyoD、肌细胞生成素和 Pax7 的表达模式。其次，还研究了间质细胞区室和肌核内肌源性标记物的表达。研究发现，与 EDL 相比，比目鱼肌含有更多数量的 Pax7、MyoD 或肌生成素阳性细胞核。类似地，与EDL相比，肌生成素在比目鱼肌的肌核中以较低水平表达，并且与EDL相比，在比目鱼肌的间质室中肌生成素以较高水平表达。当使用间质核、肌核和双标记核来估计卫星细胞群时，发现比目鱼肌横切面中大约 13% 的肌纤维和 4.1% 的 EDL 肌纤维表现出标记的卫星细胞核。总体而言，这项研究的结果表明，这些标记物的表达模式在具有不同生长动态和表型特征的肌肉中存在可预测的变化。发育动力学 235:3230–3239, 2006。© 2006 Wiley-Liss, Inc.
Satellite cells are the myogenic cells lying between the myofiber sarcolemma and basal lamina. The objective of this study was to determine the expression patterns of MyoD, myogenin, and Pax7 within the satellite cell population in the growing rat soleus and extensor digitorum longus (EDL) muscles. Secondly, the expression of the myogenic markers was also studied within the interstitial cell compartment and myonuclei. It was discovered that the soleus contained a higher number of Pax7, MyoD, or myogenin‐positive nuclei compared with the EDL. Similarly, myogenin was expressed at a lower level in the myonuclei of the soleus compared with the EDL, and myogenin was expressed at a higher level in the interstitial compartment of the soleus compared with the EDL. When interstitial nuclei, myonuclei, and double‐labeled nuclei were used in the estimate of the satellite cell population, it was discovered that approximately of 13% of the myofibers in a transverse section of the soleus muscle and 4.1% of EDL myofibers exhibit a labeled satellite cell nucleus. Overall, results from this study suggest that expression patterns of these markers vary predictably among muscles with different growth dynamics and phenotypic characteristics. Developmental Dynamics 235:3230–3239, 2006. © 2006 Wiley‐Liss, Inc.
影响因子：
2.7
作者：
Olguin, HC;Olwin, BB
通讯作者：
Olwin, BB
DOI：
10.1152/ajpcell.1998.275.4.c1124
发表时间：
1998-10-01
影响因子：
5.5
作者：
Dupont-Versteegden, EE;Houlé, JD;Peterson, CA
通讯作者：
Peterson, CA
MyoD 和肌细胞生成素 mRNA 在快速和慢速成年骨骼肌中的选择性积累受到神经支配和激素的控制。
DOI：
10.1242/dev.118.4.1137
发表时间：
1993
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Hughes,SM;Taylor,JM;Tapscott,SJ;Gurley,CM;Carter,WJ;Peterson,CA
通讯作者：
Peterson,CA