The limits of log-ratios
The limits of log-ratios
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DOI：
10.1186/1472-6750-4-3
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发表时间：
2004-03-08
影响因子：
3.5
通讯作者：
Quackenbush, J
中科院分区：
文献类型：
--
作者：
Sharov, V;Kwong, KY;Quackenbush, J
背景资料：DNA微阵列测定通常比较两个生物样品，并将这些比较的结果逐个基因地呈现为两个样品的测量的表达水平的比率的以2为底的对数。由于荧光和其他检测系统的固定动态范围，使用任何阵列技术可以进行的比较范围都有限制，在解释任何此类分析的结果时必须考虑到这一点。结论：微阵列数据收集系统的动态范围导致可从此类测量导出的比较分析中的限制，并且表明可通过进行避开微阵列数据收集系统的动态范围的边界的测量来获得最佳结果。动态范围。
Background: DNA microarray assays typically compare two biological samples and present the results of those comparisons gene-by-gene as the logarithm base two of the ratio of the measured expression levels for the two samples.Results: Because of the fixed dynamic range of fluorescence and other detection systems, there is a limit to the range of comparisons that can be made using any array technology, and this must be taken into account when interpreting the results of any such analysis.Conclusions: The dynamic range of microarray data collection systems results in limits in the comparative analyses that can be derived from such measurements and suggests that optimal results can be obtained by making measurements that avoid the boundaries of that dynamic range.