Finger-actuated, self-contained immunoassay cassettes
Finger-actuated, self-contained immunoassay cassettes
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DOI：
10.1007/s10544-009-9334-4
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发表时间：
2009-12-01
影响因子：
2.8
通讯作者：
Bau, Haim H.
中科院分区：
文献类型：
--
作者：
Qiu, Xianbo;Thompson, Jason A.;Bau, Haim H.
描述了用于廉价的、一次性的、基于发光的微流体免疫测定盒的构建块，并且证明了它们在即时诊断系统中的集成。卡匣中的流体运动通过按压手指致动袋来驱动。免疫测定所需的所有试剂都可以以液体形式储存在盒中。使用前，卡匣由两个独立部分组成。顶部储存组件包含袋、密封储存室、计量室和针座。底部处理组件包含连接针、混合室和具有固定化蛋白质的检测室。在样品引入之后，将存储和处理部件配对。针在两个部件之间形成液压连接，并且在某些情况下关闭阀门。然后依次致动袋以引起各种试剂的流动并促进过程操作。试剂卡与不同的检测模式兼容。构建并评价了基于免疫层析的检测试剂盒和基于微珠的检测试剂盒。免疫层析盒用于检测唾液样本中的HIV抗体。基于珠的盒用于检测促炎趋化因子IL-8。实验数据显示了良好的重复性和合理的灵敏度。
The building blocks for an inexpensive, disposable, luminescence-based microfluidic immunoassay cassette are described, and their integration in a point-of-care diagnostic system is demonstrated. Fluid motion in the cassette is driven by depressing finger-actuated pouches. All reagents needed for the immunoassay can be stored in the cassette in liquid form. Prior to use, the cassette consists of two separate parts. A top storage component contains pouches, sealed storage chambers, a metering chamber, and needle seats. The bottom processing component contains connection needles, a mixing chamber, and a detection chamber with immobilized proteins. Subsequent to sample introduction, the storage and processing components are mated. The needles form hydraulic connections between the two parts and, in some cases, close valves. The pouches are then actuated sequentially to induce flow of various reagents and facilitate process operations. The cassette is compatible with different detection modalities. Both a cassette with immunochromatographic-based detection and a cassette with microbead-based detection were constructed and evaluated. The immunochromatographic cassette was used to detect antibodies to HIV in saliva samples. The bead-based cassette was used to detect the proinflammatory chemokine IL-8. The experimental data demonstrates good repeatability and reasonable sensitivity.