Relationship of cell-cycle expression of Ia-like antigenic determinants on normal and leukemia human granulocyte-macrophage progenitor cells to regulation in vitro by acidic isoferritins.
Relationship of cell-cycle expression of Ia-like antigenic determinants on normal and leukemia human granulocyte-macrophage progenitor cells to regulation in vitro by acidic isoferritins.
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正常和白血病人粒细胞-巨噬细胞祖细胞上 Ia 样抗原决定簇的细胞周期表达与酸性异铁蛋白体外调节的关系。
DOI：
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发表时间：
1982
影响因子：
15.9
通讯作者：
H. Broxmeyer
中科院分区：
文献类型：
--
作者：
H. Broxmeyer
人类Ia样（Ia）抗原决定因子、粒细胞-巨噬细胞集落形成细胞（CFU-GM） DNA合成过程中的表达与酸性异铁蛋白的体外调节作用之间已经建立了联系。用单克隆抗Ia样（Ia）和补体治疗人骨髓细胞可抑制约50%的集落和簇的形成，但对cfu - gm前没有影响。无论骨髓细胞暴露于抗ia +补体、高比活性氚化胸腺嘧啶（(3)HTdr）还是酸性异铁蛋白，菌落和簇的减少都是相似的。去除ia抗原（+）CFU-GM后，(3)HTdr或酸性异铁蛋白含量均无明显下降；去除DNA合成期（s期）CFU-GM后，(3)抗ia +补体或酸性异铁蛋白含量均无明显下降。在没有补体的情况下，抗- ia不会减少菌落或簇的形成，但会阻断酸性异铁蛋白的抑制作用。Ia抗原与酸性异铁蛋白活性之间存在以下关系：(a)在37℃（而非27℃或4℃）培养5小时后，CFU-GM中ia抗原的明显丢失与对酸性异铁蛋白抑制的无反应性有关；(b)对酸性异铁蛋白不敏感的非循环前CFU-GM中ia抗原（-）可在体外产生对酸性异铁蛋白抑制有反应的ia抗原（+）循环CFU-GM群体。(c)白血病患者CFU-GM上ia抗原检测不出与酸性异铁蛋白抑制无应答性相关。这些结果暗示ia抗原（+）祖细胞在体外调节骨髓形成，并表明患者CFU-GM缺乏ia抗原与体外正常调节相互作用的不平衡有关。这些发现可能与正常调节和白血病的进展有关。
An association has been established between human Ia-like (Ia) antigenic determinants, expression during DNA synthesis on granulocyte-macrophage colony forming cells (CFU-GM) and the regulatory action of acidic isoferritins in vitro. Treatment of human bone marrow cells with monoclonal-anti-Ia-like (Ia) plus complement inhibited colony and cluster formation by approximately 50% but did not affect pre-CFU-GM. Reduction of colonies and clusters was similar whether bone marrow cells were exposed to anti-Ia plus complement, high specific activity tritiated thymidine ((3)HTdr) or acidic isoferritins. No further decrease was apparent with (3)HTdr or acidic isoferritins after Ia-antigen(+) CFU-GM were removed, or with anti-Ia plus complement or acidic isoferritins after DNA synthetic phase (S-phase) CFU-GM were removed. Anti-Ia, without complement, did not reduce colony or cluster formation but did block the inhibitory action of acidic isoferritins. A relationship existed between Ia antigens and the activity of acidic isoferritins in the following ways: (a) The apparent loss of Ia-antigens from CFU-GM by 5 h in culture at 37 degrees C, but not at 27 degrees or 4 degrees C, was associated with nonresponsiveness to inhibition with acidic isoferritins, (b) Ia-antigen(-), noncycling pre-CFU-GM that were insensitive to acidic isoferritins could generate a population of Ia-antigen(+) cycling CFU-GM in vitro that were responsive to inhibition by acidic isoferritins, and (c) nondetectability of Ia-antigens on CFU-GM from patients with leukemia was associated with nonresponsiveness to inhibition by acidic isoferritins. These results implicate Ia-antigen(+) progenitor cells in the regulation of myelopoiesis in vitro and demonstrate that absence of Ia-antigens on patient CFU-GM is associated with imbalances in normal regulatory interactions in vitro. These findings may be of relevance to normal regulation and to the progression of leukemia.
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使用单克隆抗体探针定义 HLA-DR 表位区域。
DOI：
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发表时间：
1980
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Grumet,FC;Charron,DJ;Fendly,BM;Levy,R;Ness,DB
通讯作者：
Ness,DB
人类细胞系表达多种 Ia 样分子。
影响因子：
2.7
作者：
Pesando,JM;Nadler,LM;Lazarus,H;Tomaselli,KJ;Stashenko,P;Ritz,J;Levine,H;Yunis,EJ;Schlossman,SF
通讯作者：
Schlossman,SF
鼠 B 淋巴瘤细胞系 Ia 抗原表达的细胞周期相关异质性：流式细胞术分析。
DOI：
--
发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Lanier,LL;Warner,NL
通讯作者：
Warner,NL
HLA-A、B 和 Ia 样抗原的单克隆抗体可抑制人骨髓祖细胞的集落形成。
DOI：
--
发表时间：
1980
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Fitchen,JH;Ferrone,S;Quaranta,V;Molinaro,GA;Cline,MJ
通讯作者：
Cline,MJ
DOI：
--
发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Beller,DI;Unanue,ER
通讯作者：
Unanue,ER