Detection of viruses directly from the fresh leaves of a Phalaenopsis orchid using a microfluidic system
Detection of viruses directly from the fresh leaves of a Phalaenopsis orchid using a microfluidic system
复制标题
DOI：
10.1016/j.nano.2013.05.016
复制
发表时间：
2013-11-01
影响因子：
5.4
通讯作者：
Lee, Gwo-Bin
中科院分区：
文献类型：
--
作者：
Chang, Wen-Hsin;Yang, Sung-Yi;Lee, Gwo-Bin
病原体的早期检测对于有效监测疾病至关重要。已经做出了许多努力来探索可以在短时间内检测到这些病原体的方法，而不需要繁琐的程序。然而，这些开发的方法有缺点，如它们相对耗时或需要专门的实验室设施。在这项工作中，我们开发了一个集成的微流控系统，通过直接分析从新鲜的蝴蝶兰叶片中快速和自动地检测病毒。整个程序，包括核糖核酸(RNA)提纯、逆转录环介导恒温扩增(RT-LAMP)和通过测量浊度变化进行光学检测，都在一个芯片上完成。这是首次展示了用于检测感染蝴蝶兰的病毒的集成微流控系统。本研究还探讨了所开发系统的灵敏度，以验证其性能。来自临床编辑：在本研究中，作者报告了一种集成的微流控系统的开发，该系统通过直接分析新鲜蝴蝶兰叶片来快速和自动检测病毒，使用单芯片执行三步程序。类似的方法可能会在临床上应用于快速准确地检测病毒感染。(C)2013 Elsevier Inc.保留所有权利。
Early detection of pathogens is crucial for the effective surveillance of diseases. Many efforts have been made to explore methods which can detect these pathogens within a short period of time without requiring a tedious protocol. However, these developed methods have disadvantages such as they are relatively time-consuming or require specialized laboratory facilities. In this work, we have developed an integrated microfluidic system for rapid and automatic detection of viruses by direct analysis from fresh Phalaenopsis orchid leaves. The entire protocol, including ribonucleic acid (RNA) purification, reverse transcription loop-mediated-isothermal-amplification (RT-LAMP) and optical detection by measuring changes in turbidity was performed on a single chip. This is the first time that an integrated microfluidic system for the detection of viruses infecting the Phalaenopsis orchid has been demonstrated. The sensitivity of the developed system was also explored in this study to validate its performance.From the Clinical Editor: In this study, the authors report the development of an integrated microfluidic system for rapid and automatic detection of viruses by direct analysis of fresh Phalaenopsis orchid leaves, performing the 3-step protocol using a single chip. Similar methods may find clinical application for fast and accurate detection of viral infections. (C) 2013 Elsevier Inc. All rights reserved.