EVIDENCE OF SEQUENTIAL REMODELING IN RAT TRABECULAR BONE - MORPHOLOGY, DYNAMIC HISTOMORPHOMETRY, AND CHANGES DURING SKELETAL MATURATION
EVIDENCE OF SEQUENTIAL REMODELING IN RAT TRABECULAR BONE - MORPHOLOGY, DYNAMIC HISTOMORPHOMETRY, AND CHANGES DURING SKELETAL MATURATION
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DOI：
10.1002/ar.1092080114
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发表时间：
1984-01-01
影响因子：
--
通讯作者：
VIGNERY, A
中科院分区：
文献类型：
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作者：
BARON, R;TROSS, R;VIGNERY, A
在大鼠松质骨的次级松质层水平上，证实了与在人骨中观察到的相似的连续骨重建活动的发生。一个完整的动态组织形态学分析的重塑活动，使用未脱钙部分和双荧光标记，因此在年轻的成年人（220克，8周龄）和更成熟的动物（320克，12周龄）。尽管骨小梁体积相似，但年轻动物的骨形成率和破骨细胞比成熟动物高5倍。较高的骨形成率部分是由于双标记骨小梁表面的程度高3倍，部分是由于矿化速率快1.5倍。因此，这些结果表明大鼠骨骼成熟期间骨转换显着减慢。在这项研究中获得的值进行了比较，在同一物种或人类的骨骼的其他部分的测量。10周龄大鼠的转换率与成年人髂嵴骨小梁中观察到的值非常相似。因此，大鼠是研究骨小梁重塑的良好实验动物，但由于骨骼成熟期间发生较大变化，因此应注意选择与所问问题类型相关的年龄组。
The occurrence of a sequential bone remodeling activity, similar to what is observed in human bone, is demonstrated in rat trabecular bone at the level of the secondary spongiosa. A complete dynamic histomorphometric analysis of the remodeling activity, using undecalcified sections and double fluorescent labels, was consequently performed in young adult (220 g, 8 wk old) and in more mature animals (320 g, 12 wk old). Despite a similar trabecular bone volume, younger animals had a 5 times higher bone formation rate and 5 times more osteoclasts than more mature animals. The higher bone formation rate was due in part to a 3-fold higher extent of double-labeled trabecular bone surface and in part to a 1.5-fold faster mineralization rate. These results therefore demonstrate a marked slowing down of bone turnover during skeletal maturation in the rat. The values obtained in this study have been compared with measurements made in other parts of the skeleton in the same species or in humans. Twelve-week-old rats had a turnover rate very similar to values observed in iliac crest trabecular bone in adult humans. The rat is therefore a good experimental animal for the study of trabecular bone remodeling but since large variations occur during skeletal maturation, care should be taken in the selection of an age group relevant to the type of questions being asked.