Chronic hepatitis D virus (HDV) infection in hepatitis B virus carrier chimpanzees experimentally superinfected with HDV.
Chronic hepatitis D virus (HDV) infection in hepatitis B virus carrier chimpanzees experimentally superinfected with HDV.
复制标题
实验性重复感染乙型肝炎病毒携带者黑猩猩的慢性丁型肝炎病毒 (HDV) 感染。
DOI：
10.1093/infdis/158.1.151
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Gerin,JL
中科院分区：
文献类型：
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作者：
Negro,F;Bergmann,KF;Baroudy,BM;Satterfield,WC;Popper,H;Purcell,RH;Gerin,JL
携带慢性乙型肝炎病毒和多次感染丁型肝炎病毒（HDV）的黑猩猩显然会患上急性自限性丁型肝炎。然而，使用针对HDV基因组的RNA探针进行的基于杂交的灵敏检测表明，在接种后平均2.4 y（范围1-6.4 y），这些动物中有50%仍有可检测到的持续HDV复制迹象。检测HDV存在的唯一阳性标记是血清HDV RNA；免疫印迹法和免疫荧光法分别在血清和肝脏中检测不到HDV抗原。由于检测到的病毒基因组数量非常低，先前未能确定黑猩猩的慢性HDV携带者状态可归因于先前描述的测定方法的灵敏度较低。
Chimpanzees that were chronic hepatitis B virus carriers and that were superinfected with hepatitis D virus (HDV) apparently developed acute, self-limited type D hepatitis. Reevaluation with a sensitive hybridization-based assay using RNA probes specific for the HDV genome, however, demonstrated that >50% of these animals still had detectable signs of ongoing HDV replication an average of 2.4 y (range, 1–6.4 y) after inoculation. The only positive marker for the presence of HDV was serum HDV RNA; HDV antigen was undetectable in both serum and the liver by an immunoblot assay and immunofluorescence, respectively. Because the detected amount of viral genome was very low, the previous failure to identify the chronic HDV carrier state in the chimpanzee can be attributed to the lower sensitivity of previously described assays.