Wide mass range scanning for the fast atom bombardment mass spectrometry of very large compounds
Wide mass range scanning for the fast atom bombardment mass spectrometry of very large compounds
复制标题
用于超大化合物快速原子轰击质谱分析的宽质量范围扫描
DOI：
10.1021/ac00284a070
复制
发表时间：
1985
影响因子：
7.4
通讯作者：
C. Fenselau
中科院分区：
文献类型：
--
作者：
R. Cotter;B. Larsen;D. Heller;J. Campana;C. Fenselau
我们注意到，许多关于重量超过5000 amu的化合物的FAB质谱的出版物仅报告了分子离子区域（1，2）。这有几个原因。首先，迄今为止研究的超大型化合物都具有已知的分子量，因此可以事先选择要扫描的区域。其次，低信号水平通常要求使用信号平均技术，当在大质量范围上应用并涉及多次扫描时，该技术对于许多数据系统来说可能是麻烦的，并且可能超过样品寿命或要求多次样品加载。对于未知和高分子量的样品，快速测量分子离子和主要碎片离子区域的宽质量范围可能是有用的。对于高分辨率的双聚焦扇形仪器，我们使用了一种时间分箱技术，这种技术对于突出光谱的主要特征是有用的。
We note that many publications on the FAB mass spectra of compounds weighing more than 5000 amu report only the molecular ion region (1, 2). There are a number of reasons for this. First, the verylarge compounds studied thusfar have had known molecular weights so that it has been possible to select a priori the region which will be scanned. Secondly, low signal levels often demand that signal averaging techniques be used which, when applied over a large mass range and involving multiple scans, may be cumbersome for many data systems and may exceed the sample lifetime or demand multiple sample loadings. For samples of unknown and high molecular weight, it may be useful to rapidly survey a wide mass range for molecular ion and major fragment ion regions. For high-resolution double-focusing sector instruments, we have used a time binning technique which is useful for accentuating the major features of the spectrum.
采用高效液相色谱法进一步纯化表皮生长因子。
影响因子：
2.9
作者：
Matrisian,LM;Larsen,BR;Finch,JS;Magun,BE
通讯作者：
Magun,BE