Molecular and Cellular Dynamics of Aortic Aneurysms Revealed by Single-Cell Transcriptomics.
Molecular and Cellular Dynamics of Aortic Aneurysms Revealed by Single-Cell Transcriptomics.
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DOI：
10.1161/atvbaha.121.315852
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发表时间：
2021-11
期刊：
影响因子：
--
通讯作者：
Shen YH
中科院分区：
文献类型：
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作者：
Li Y;LeMaire SA;Shen YH
主动脉是高度异质性的，包含许多不同类型的细胞，这些细胞执行复杂的功能以维持主动脉稳态。最近，单细胞RNA测序（scRNA-seq）研究为血管细胞类型的异质性、每种细胞类型的综合分子特征以及这些细胞群体之间的表型相互关系提供了大量新的见解。这一新的信息大大提高了我们在分子和细胞水平上对主动脉生物学和动脉瘤的理解。在这里，我们总结了这些发现，重点是scRNA-seq分析揭示了细胞异质性，细胞转换，细胞群之间的通信以及血管壁中的关键转录因子。我们还回顾了从scRNA-seq中学到的信息，这些信息有助于我们了解血管疾病的发病机制，例如鉴定血管紧张素相关基因和遗传变异发挥作用的细胞类型。最后，我们讨论了scRNA-seq应用于主动脉生物学和疾病研究的挑战和未来方向。
The aorta is highly heterogeneous, containing many different types of cells that perform sophisticated functions to maintain aortic homeostasis. Recently, single-cell RNA sequencing (scRNA-seq) studies have provided substantial new insight into the heterogeneity of vascular cell types, the comprehensive molecular features of each cell type, and the phenotypic interrelationship between these cell populations. This new information has significantly improved our understanding of aortic biology and aneurysms at the molecular and cellular level. Here, we summarize these findings, with a focus on what scRNA-seq analysis has revealed about cellular heterogeneity, cellular transitions, communications among cell populations, and critical transcription factors in the vascular wall. We also review the information learned from scRNA-seq that has contributed to our understanding of the pathogenesis of vascular disease, such as the identification of cell types in which aneurysm-related genes and genetic variants function. Finally, we discuss the challenges and future directions of scRNA-seq applications in studies of aortic biology and diseases.