Inhibition of cell proliferation, migration and invasion by DNAzyme targeting MMP-9 in A549 cells.
Inhibition of cell proliferation, migration and invasion by DNAzyme targeting MMP-9 in A549 cells.
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DOI：
10.3892/or_00000414
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发表时间：
2009-07
期刊：
影响因子：
4.2
通讯作者：
Lifang Yang;Weizhong Zeng;Dan Li;R. Zhou
中科院分区：
文献类型：
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作者：
Lifang Yang;Weizhong Zeng;Dan Li;R. Zhou
基质金属蛋白酶（MMP）被认为是在血管生成和转移过程中协助肿瘤细胞的主要关键分子。基质金属蛋白酶-9 (MMP-9) 表达增强与人类非小细胞肺癌 (NSCLC) 侵袭和转移相关。 DNAzyme 是一种单链 DNA 催化剂，经过改造后可与其靶基因中的互补序列结合并切割 mRNA。本研究设计并合成了靶向MMP-9的DNAzyme。我们发现它强烈抑制 NSCLC 细胞系 A549 中的 MMP-9 mRNA 和蛋白表达。还证明了对细胞增殖、粘附、迁移和侵袭的抑制作用。因此，靶向MMP-9的DNAzyme可能是NSCLC癌症基因治疗的一种有前途的抗侵袭和抗转移策略。
Matrix metalloproteinases (MMPs) have been regarded as major critical molecules assisting tumor cells during angiogenesis and metastasis. Enhanced expression of matrix metalloproteinase-9 (MMP-9) is associated with human non-small cell lung cancer (NSCLC) invasion and metastasis. DNAzyme is a single-stranded DNA catalyst that can be engineered to bind to its complementary sequence in the target gene and cleave the mRNA. In this study, DNAzyme targeting MMP-9 was designed and synthesized. We found it strongly inhibited MMP-9 mRNA and protein expression in the NSCLC cell line A549. Inhibition of cell proliferation, adhesion, migration and invasion was also demonstrated. Thus, DNAzyme targeting MMP-9 may be a promising anti-invasion and anti-metastasis strategy for cancer gene therapy in NSCLC.