Immunolocalization of androgen receptors and aromatase enzyme in the adult musk shrew brain.
Immunolocalization of androgen receptors and aromatase enzyme in the adult musk shrew brain.
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成年麝香鼩脑中雄激素受体和芳香酶的免疫定位。
DOI：
10.1159/000054568
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发表时间：
2000
影响因子：
4.1
通讯作者：
Rissman,EF
中科院分区：
文献类型：
--
作者：
Veney,SL;Rissman,EF
在大脑和其他组织中，雌激素是通过雄激素的芳构化产生的。生化数据表明芳香化酶受雄激素受体（AR）调节。含有AR或芳香酶（AROM）的神经元存在于许多相同的大脑区域。在这份报告中，我们研究了共分布的AR和AROM酶免疫反应（IR）神经元在几个地区的成年男性和女性麝香？数据收集自外侧隔中间核（LS）、终纹床核内侧前（BNSTMA）和内侧后内侧（BNSTMP）、内侧视前区（mPOA）、下丘脑腹内侧核（VMN）、杏仁核内侧核（MeA）、皮质核和中央核。雄性大鼠BNSTMP、mPOA、VMN和LS的AR阳性神经元显著多于雌性大鼠。除BNSTMA和LS外，男性在每个区域都比女性有更多的AROM-ir神经元。此外，在BNSTMP，mPOA，VMN，LS和MeA中，男性的双标记神经元明显多于女性。AROM-IR神经元也含有AR免疫反应性的百分比范围为13%至82%，这取决于性别和地区。双标记神经元的比例最高（女性79%，男性82%）被发现在VMN。两者合计，这些数据表明，有广泛的AR和AROM酶的细胞共定位在特定区域的麝香鼩脑。我们认为，在两性中，雄激素受体可能作为转录因子调节AROM酶。
In the brain and other tissues, estrogens are produced by aromatization of androgens. Biochemical data suggest that aromatase enzyme is regulated by the androgen receptor (AR). Neurons that contain either AR or aromatase (AROM) enzyme reside in many of the same brain regions. In this report, we examined the codistribution of AR- and AROM-enzyme-immunoreactive (-ir) neurons in several regions of the adult male and female musk shrew brain. Data were collected from the intermediate nucleus of the lateral septum (LS), medial anterior (BNSTMA) and medial posterointerior (BNSTMP) divisions of the bed nucleus of the stria terminalis, medial preoptic area (mPOA), ventromedial nucleus of the hypothalamus (VMN), medial (MeA), cortical and central nuclei of the amygdala. Males had significantly more AR-ir neurons in the BNSTMP, mPOA, VMN and LS as compared to females. With the exception of the BNSTMA and LS, males had more AROM-ir neurons in each region than females. Furthermore, males had significantly more double-labeled neurons than females in the BNSTMP, mPOA, VMN, LS and MeA. The percentage of AROM-ir neurons that also contained AR immunoreactivity ranged from 13 to 82% depending on sex and region. The highest percentage of dual-labeled neurons (79% in females and 82% in males) was found in the VMN. Taken together, these data show that there is extensive cellular colocalization of AR and AROM enzyme in specific regions of the musk shrew brain. We propose that in both sexes, androgen receptors may act as transcription factors to regulate AROM enzyme.