TLR2, TLR3, and TLR4 activation specifically alters the oxidative status of intestinal epithelial cells
TLR2, TLR3, and TLR4 activation specifically alters the oxidative status of intestinal epithelial cells
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DOI：
10.1007/s12192-013-0461-8
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发表时间：
2014-03-01
影响因子：
3.8
通讯作者：
Mesonero, Jose E.
中科院分区：
文献类型：
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作者：
Latorre, Eva;Mendoza, Carmen;Mesonero, Jose E.
肠炎性疾病是多种过程的结果，包括粘膜氧化应激和共生细菌与粘膜免疫之间动态平衡的紊乱。Toll样受体(Toll-like Receptor，TLRs)识别分子相关微生物的模式，并触发先天免疫反应，有助于肠道内稳态和炎症反应。然而，TLRs对肠粘膜氧化还原平衡的影响尚不清楚。因此，本研究分析了TLR2、TLR3和TLR4对肠上皮样Caco-2细胞脂质和蛋白质氧化损伤以及抗氧化酶活性的影响。结果表明，这些TLR的激活增加了脂质和蛋白质的氧化水平，但对抗氧化酶活性的影响因TLR激活的不同而不同。这些结果表明，TLR2、TLR3和TLR4的激活可能不仅通过其固有的免疫反应影响肠道炎症，而且可能通过其对肠上皮细胞的促氧化作用来影响肠道炎症。
Intestinal inflammatory diseases are the result of multiple processes, including mucosal oxidative stress and perturbed homeostasis between commensal bacteria and mucosal immunity. Toll-like receptors (TLRs) recognize molecular-associated microorganisms' patterns and trigger innate immunity responses contributing to intestinal homeostasis and inflammatory responses. However, TLRs effects on redox balance in intestinal mucosa remain unknown. Therefore, the present study analyzes the effect of TLR2, TLR3, and TLR4 on both oxidative damage of lipids and proteins, and the activity of antioxidant enzymes in enterocyte-like Caco-2 cells. The results show that the activation of these TLRs increased lipid and protein oxidation levels; however, the effect on the antioxidant enzymes activity is different depending on the TLR activated. These results suggest that the activation of TLR2, TLR3, and TLR4 might affect intestinal inflammation by not only their inherent innate immunity responses, but also their pro-oxidative effects on intestinal epithelial cells.