Radiation response of primary human skin fibroblasts and their bystander cells after exposure to counted particles at low and high LET
Radiation response of primary human skin fibroblasts and their bystander cells after exposure to counted particles at low and high LET
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DOI：
10.1080/09553000600582979
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发表时间：
2006-01-01
影响因子：
2.6
通讯作者：
Giesen, U
中科院分区：
文献类型：
--
作者：
Frankenberg, D;Greif, KD;Giesen, U
目的：为了研究低剂量区线性能量传递（LET）对旁观者效应的影响，采用德国联邦物理技术研究院（PTB）的单离子微束照射融合的原代人皮肤成纤维细胞。用10 MeV质子（LET约为4.7 keV/μ m）和4.5 MeV α粒子（LET约为100 keV/μ m）靶向接种在特殊辐照皿上的细胞。在曝光过程中，细胞融合，允许通过两个间隙连接和diffusion.Results的信号传输：10 MeV的质子的克隆形成能力显着高于暴露后70质子（0.31戈伊）与未照射的细胞相比。对于更高剂量，存活曲线是指数的。只有10%的所有核暴露在低剂量区导致类似的辐射反应。对于高达2.2戈伊的较高剂量，未观察到细胞杀伤。对于4.5 MeV α粒子，获得了指数存活曲线。只有10%的所有细胞核的照射导致的存活曲线已预期在没有任何旁观者effect.Conclusion：旁观者效应的类型和程度原来是依赖于粒子的LET，并可能也取决于所使用的细胞系和所应用的技术。
Purpose: To investigate the dependence of bystander effects on linear energy transfer (LET) in the low dose region.Materials and Methods: The single-ion microbeam of the Physikalisch-Technische Bundesanstalt (PTB) was used to irradiate confluent primary human skin fibroblasts. Cells plated on a special irradiation dish were targeted with 10 MeV protons (LET approximate to 4.7 keV/mu m) and 4.5 MeV alpha-particles (LET approximate to 100 keV/mu m). During exposure, the cells were confluent allowing signal transfers through both gap junctions and diffusion.Results: For 10 MeV protons the clonogenic capability was significantly higher after exposure to 70 protons (0.31 Gy) compared with unirradiated cells. For higher doses the survival curve was exponential. Exposure of only 10% of all nuclei resulted in a similar radiation response in the low dose region. For higher doses up to 2.2 Gy no cell killing was observed. For 4.5 MeV alpha-particles an exponential survival curve was obtained. Irradiation of only 10% of all cell nuclei resulted in an survival curve as had been expected in the absence of any bystander effect.Conclusion: The type and extent of bystander effects turned out to be dependent on the particles' LET and are likely to depend also on the cell line used and the techniques applied.