Prolonged rat cardiac preservation with UW lactobionate solution.
Prolonged rat cardiac preservation with UW lactobionate solution.
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发表时间：
1989-02
影响因子：
0.9
通讯作者：
L. Makowka;Tony R. Zerbe;F. Chapman;S. Qian;Hong Sun;N. Murase;Robert L. Kormos;James Snyder;T. Starzl
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作者：
L. Makowka;Tony R. Zerbe;F. Chapman;S. Qian;Hong Sun;N. Murase;Robert L. Kormos;James Snyder;T. Starzl
进一步扩大临床心脏移植的最重要限制因素包括合适供体的可用性和器官在收获后的存活时间。目前，使用表1中列出的标准斯坦福大学保存溶液，静态冷心脏保存的安全限度被认为是4至6小时。Belzer及其同事开发了一种保存溶液，称为威斯康星州大学（UW）乳糖酸盐冷藏溶液（表1），该溶液已证明胰腺和肝脏分别可持续保存72小时和30小时或更长时间。我们在大鼠异位心脏移植模型中比较了UW-溶液和传统的斯坦福大学溶液用于心脏保存。评估了使用血小板激活因子（PAF）拮抗剂（已被证明可以改善缺血性肝损伤）2和氧自由基清除剂（SOD）的其他药理学操作。表1冷藏溶液组成
The most significant limiting factor in the further expansion of clinical cardiac transplantation consists of the availability of suitable donors and the duration of viability of the organ following harvest. Currently, the safe limits of static cold cardiac preservation are considered to be four to six hours using the standard Stanford preservation solution as listed in Table 1. Belzer and colleagues have developed a preservation solution known as the University of Wisconsin (UW) lactobionate cold-storage solution (Table 1) which has demonstrated consistent 72 hour and 30 hour or longer preservation of the pancreas and liver, respectively.1 Prompted by these successes and significant advances in static cold preservation, we compared UW-solution and the traditional Stanford solution for cardiac preservation in a heterotopic heart transplant model in rats. Additional pharmacologic manipulation with an antagonist of Platelet-Activating Factor (PAF), which has been shown to ameliorate ischemic liver injury,2 and with an oxygen free radical scavenger (SOD) were evaluated. Table 1 Composition of Cold Storage Solutions