In vitro propagation using adventitious buds technique as a source of new variability in chrysanthemum
In vitro propagation using adventitious buds technique as a source of new variability in chrysanthemum
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使用不定芽技术的体外繁殖作为菊花新变异的来源
DOI：
10.1016/j.scienta.2007.01.019
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发表时间：
2007
影响因子：
4.3
通讯作者：
N. Miler
中科院分区：
文献类型：
--
作者：
M. Zalewska;J. Lema;N. Miler
Chrysanthemum×grandiflorum的11个品种（拉马特）Kitam。：“Richmond”及其10个辐射突变体，代表Lady组，以茎尖和叶片为外植体进行离体繁殖。本研究的目的是探讨用于微繁的外植体类型是否影响菊花的基因型和表型。从茎尖和叶片上形成的不定芽生长的植株离体生根，在温室中驯化和培养，直到开花。比较了两种不同外植体类型的植株花序的颜色和形状。所有由茎尖外植体获得的植株均表现出该品种典型的花序颜色和形状。“Richmond”、“Lady Amber”、“Lady White”和“Lady Yellow”四个品种的不定芽衍生植物的花序颜色都是真实的。所有用不定芽技术繁殖的“女杏”（原：金甜菜）和“女鲑鱼”（鲑鱼）的花序颜色都发生了变化（分别为紫金、粉红色和白色）。用不定芽繁殖的“青铜夫人”（原：红棕色）、“橙色夫人”（橙棕色）和“玫瑰色夫人”（紫金色）的花序颜色都是典型的，也发生了变化（分别为黄色、黄色和红色、红粉色）。当用茎尖和叶子作为外植体繁殖时，‘Vitroflora夫人’的舌状小花在花序上长成管状花序的数量发生了变化。这些变化可能是植物嵌合结构或体细胞无性系变异的影响。这种变异只有在使用非分生外植体时才会出现。不定芽技术作为一种新的变异源，在菊花育种中可能是有用的。
Eleven cultivars of Chrysanthemum×grandiflorum (Ramat.) Kitam.: ‘Richmond’ and its 10 radiomutants, representing the Lady group, were propagated in vitro with shoot tips and leaves as explants. The aim of this study was to investigate if the explant type used for micropropagation affects the genotype and phenotype of chrysanthemums. Plants grown from shoot tips and adventitious buds formed on leaves were rooted in vitro, acclimatized and cultivated in glasshouse up to full-flowering. The colour and shape of inflorescences of plants obtained from two different explant types were compared within the cultivars. All plants derived from shoot-tip explants showed the inflorescence colour and shape typical for the cultivars. Inflorescence colour of plants derived from adventitious buds were true-to-type in four cultivars: ‘Richmond’, ‘Lady Amber’, ‘Lady White’ and ‘Lady Yellow’. All plants of ‘Lady Apricot’ (originally: golden beet) and ‘Lady Salmon’ (salmon) propagated from adventitious buds technique showed altered inflorescence colour (respectively: purple gold; pink and white). ‘Lady Bronze’ (originally: reddish brown), ‘Lady Orange’ (orange brown) and ‘Lady Rosy’ (purple gold) propagated with adventitious buds had both typical and changed inflorescence colours (respectively: yellow; yellow and red; reddish pink). ‘Lady Vitroflora’ showed altered number of ligulate florets grown into tubes in inflorescence when propagated with shoot tips and leaves as explants. Those changes might be an effect of either chimeral structure or somaclonal variation of the plants investigated. The variation appears only if non-meristematical explants were used. The adventitious buds technique might be useful in chrysanthemum breeding as a source of a new variability.