Polar production of interleukin-8 by mesothelial cells promotes the transmesothelial migration of neutrophils: role of intercellular adhesion molecule-1.
Polar production of interleukin-8 by mesothelial cells promotes the transmesothelial migration of neutrophils: role of intercellular adhesion molecule-1.
复制标题
间皮细胞极性产生白介素 8 促进中性粒细胞跨间皮迁移：细胞间粘附分子 1 的作用。
DOI：
10.1086/320700
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Antony,VB
中科院分区：
文献类型：
--
作者：
Nasreen,N;Mohammed,KA;Hardwick,J;VanHorn,RD;Sanders,KL;Doerschuk,CM;Hott,JW;Antony,VB
Migration of polymorphonuclear neutrophils (PMNL) from the vascular compartment into the pleural space occurs rapidly during the development of parapneumonic effusions. This study investigated the polarized secretion of interleukin (IL)–8 in activated pleural mesothelial cells (PMC) and the migration of PMNL across resting, activated PMC monolayers. Results show that PMC produce IL-8 in a polar manner. When PMC were stimulated withStaphylococcus aureusor IL-1β at the basal or at the apical surface, significantly (P<.05) more IL-8 was released toward the apical surface. This polarized production of IL-8 was confirmed by in situ hybridization. PMNL migration was higher from the basilar to apical than from the apical to basilar surface of PMC. Neutralizing antibodies against IL-8 and intercellular adhesion molecule (ICAM)–1 significantly (P<.001) blocked PMNL migration across activated monolayers. Thus, during pleural inflammation, PMC regulate the influx of PMNL into the pleural space by polar production of IL-8 and expression of ICAM-1
