Highly multiplexed imaging of tumor tissues with subcellular resolution by mass cytometry
Highly multiplexed imaging of tumor tissues with subcellular resolution by mass cytometry
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DOI：
10.1038/nmeth.2869
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发表时间：
2014-04-01
期刊：
影响因子：
48
通讯作者：
Bodenmiller, Bernd
中科院分区：
文献类型：
--
作者：
Giesen, Charlotte;Wang, Hao A. O.;Bodenmiller, Bernd
质谱流式细胞术能够对细胞类型和状态进行高维单细胞分析。在质谱流式细胞术中，稀土金属被用作抗体上的报告基因。使用质谱流式细胞仪分析金属丰度允许测定单个细胞中的标记物表达。质谱流式细胞术以前仅应用于细胞悬液。为了获得空间信息，我们将免疫组织化学和免疫细胞化学方法与高分辨率激光消融耦合到CyTOF质谱细胞术。这种方法能够同时成像的32种蛋白质和蛋白质修饰在亚细胞分辨率;与额外的同位素的可用性，超过100个标记物的测量将是可能的。我们将成像质谱细胞术应用于人类乳腺癌样本，从而描绘细胞亚群和细胞-细胞相互作用并突出肿瘤异质性。成像质谱细胞术补充了现有的成像方法。它将使组织异质性和功能的基础研究成为可能，并支持医学向个体化分子靶向诊断和治疗的过渡。
Mass cytometry enables high-dimensional, single-cell analysis of cell type and state. In mass cytometry, rare earth metals are used as reporters on antibodies. Analysis of metal abundances using the mass cytometer allows determination of marker expression in individual cells. Mass cytometry has previously been applied only to cell suspensions. To gain spatial information, we have coupled immunohistochemical and immunocytochemical methods with high-resolution laser ablation to CyTOF mass cytometry. This approach enables the simultaneous imaging of 32 proteins and protein modifications at subcellular resolution; with the availability of additional isotopes, measurement of over 100 markers will be possible. We applied imaging mass cytometry to human breast cancer samples, allowing delineation of cell subpopulations and cell-cell interactions and highlighting tumor heterogeneity. Imaging mass cytometry complements existing imaging approaches. It will enable basic studies of tissue heterogeneity and function and support the transition of medicine toward individualized molecularly targeted diagnosis and therapies.