ROLE OF DIVALENT CATIONS IN ACTION OF POLYMYXIN-B AND EDTA ON PSEUDOMONAS-AERUGINOSA
ROLE OF DIVALENT CATIONS IN ACTION OF POLYMYXIN-B AND EDTA ON PSEUDOMONAS-AERUGINOSA
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DOI：
10.1099/00221287-59-2-263
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发表时间：
1969-01-01
期刊：
影响因子：
--
通讯作者：
MELLING, J
中科院分区：
文献类型：
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作者：
BROWN, MRW;MELLING, J
铜绿假单胞菌在缺镁条件下分批培养时，对多粘菌素B失去敏感性，产生裂解，释放260 nm的荧光。吸收材料和丧失生存能力。多粘菌素产生的裂解和泄漏的模式是相似的，在不同的Mg浓度和高多粘菌素浓度的抑制作用的生长的影响。任何一种混悬液的溶解和渗漏率相似。不敏感细菌对多粘菌素的摄取减少。此外，镁或几种阳离子之一恢复敏感性多粘菌素和EDTA在不同程度上，但只有经过几次细胞分裂发生。一个密切的相似性之间的影响，镁耗尽对EDTA和多粘菌素的敏感性，它们的作用机制之间的关系建议。有人提出，阳离子是必不可少的合成的敏感组件的信封，本身可能参与的组件的结构。
Pseudomonas aeruginosagrown under conditions of Mg-depletion in batch eulture in simple salts medium lost sensitivity to polymyxin B, causing lysis, release of 260 nm. absorbing materials and loss of viability. The patterns of lysis and leakage produced by polymyxin were similar with regard to the effect of growth in different Mg concentrations and the inhibitory effect of high polymyxin concentrations. The rates of lysis and leakage for any one suspension were similar. There was decreased polymyxin uptake by insensitive bacteria. Addition of Mg or one of several cations restored sensitivity both to polymyxin and to EDTA to varying degrees, but only after several cell divisions had occurred. A close similarity was observed between the effects of Mg-depletion on sensitivity to EDTA and to polymyxin; a relationship between their mechanisms of action is suggested. It is proposed that cations are essential for the synthesis of sensitive components of the envelope and may themselves be involved in the structure of the component.