Characterization of 17β-hydroxysteroid dehydrogenase type 7 in reproductive tissues of the marmoset monkey
Characterization of 17β-hydroxysteroid dehydrogenase type 7 in reproductive tissues of the marmoset monkey
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DOI：
10.1095/biolreprod.102.012476
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发表时间：
2003-06-01
影响因子：
3.6
通讯作者：
Einspanier, A
中科院分区：
文献类型：
--
作者：
Husen, B;Adamski, J;Einspanier, A
与已知的啮齿动物酶相比，灵长类动物中17 β -羟基类固醇脱氢酶7型（17HSD7）的生理意义及其在生殖生物学中的功能尚不清楚。作为第一步，我们最近克隆了人类和狨猴（Callithrix jacchus） 17HSD7 （cj17HSD7）的完整编码区。本研究通过对绒猴17HSD1 （cj17HSD1）全长cDNA（包括近端启动子区）和绒猴芳香化酶（cjARO）部分序列的测序，比较了这些雌二醇合成酶与绒猴17HSD7在灵长类动物模型中的表达，并确定了17HSD7可能参与雌二醇合成途径的组织。测定了cj17HSD1和cj17HSD7的基因结构，证实其与人类同源基因非常相似。Northern杂交表明，cjARO mRNA在胎盘中与cj17HSD1共表达较好，而在其他组织中仅与cj17HSD7平行表达。特别是在黄体中，cj17HSD7转录在卵巢周期的整个黄体期都可以检测到，并在怀孕期间增加，与芳香化酶的转录平行。采用新的cj17HSD7和cjARO蛋白多克隆抗血清进行免疫印迹和免疫组化检测。研究了绒猴黄体中雌酮转化为雌二醇的酶促作用。与芳香化酶的共表达模式支持了黄体17HSD7与胎盘17HSD1互补的假设，确保了灵长类动物在妊娠期间持续的雌二醇合成。
In contrast to the known rodent enzymes, the physiological significance of 17beta-hydroxysteroid dehydrogenase type 7 (17HSD7) and its presumed function in reproductive biology is not well understood in primates. As a first step, we recently cloned the complete coding regions of human and marmoset monkey (Callithrix jacchus) 17HSD7 (cj17HSD7). In the present work the complete cDNA of marmoset 17HSD1 (cj17HSD1), including the proximal promoter region, and a partial sequence of marmoset aromatase (cjARO) were sequenced in order to compare the expression of these estradiol synthesizing enzymes with that of 17HSD7 in a primate model and to identify tissues where 17HSD7 might participate in the pathway of estradiol synthesis. The gene structures of cj17HSD1 and cj17HSD7 were determined and proved to be very similar to the human orthologues. Northern hybridization showed that cjARO mRNA seems to be coexpressed preferably with cj17HSD1 in placenta, whereas in other tissues it is expressed in parallel only with cj17HSD7. Especially in corpora lutea, the cj17HSD7 transcript is detectable throughout the luteal phase of the ovarian cycle and increases during pregnancy, in parallel with the transcript of aromatase. Results were confirmed by immunoblots and immunohistochemistry using new polyclonal antisera directed against cj17HSD7 and cjARO protein. The enzymatic conversion of estrone to estradiol was assessed in marmoset corpora lutea. The pattern of coexpression with aromatase supports the hypothesis that luteal 17HSD7 complements placental 17HSD1, ensuring continued estradiol synthesis throughout pregnancy in primates.