Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors
Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors
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DOI：
10.1002/ar.1128
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发表时间：
2001-09-01
影响因子：
--
通讯作者：
Black, AC
中科院分区：
文献类型：
--
作者：
Young, HE;Steele, TA;Black, AC
本研究对人结缔组织来源的储备干细胞的13种细胞表面簇分化标志物进行了研究。干细胞分离自来自胎儿、成熟和老年人的真皮和骨骼肌的结缔组织。使用胰岛素/地塞米松表型生物测定来确定来自每个群体的干细胞的身份。所有群体均含有谱系定向的成肌、成脂、成软骨和成骨祖干细胞以及能够形成肌肉、脂肪细胞、软骨、骨、成纤维细胞和内皮细胞的谱系非定向多能干细胞。成人干细胞群的流式细胞术分析显示CD 34和CD 90阳性染色，CD 3、CD 4、CD 8、CD 11 c、CD 33、CD 36、CD 38、CD 45、CD 117、血型糖蛋白-A和HLA DR-II阴性染色。Anat Rec 264：51- 62，2001。(C)2001 Wiley-Liss，Inc.
This study details the profile of 13 cell surface cluster differentiation markers on human reserve stem cells derived fi-om connective tissues. Stem cells were isolated from the connective tissues of dermis and skeletal muscle derived from fetal, mature, and geriatric humans. An insulin/dexamethasone phenotypic bioassay was used to determine the identity of the stem cells from each population. All populations contained lineage-committed myogenic, adipogenic, chondrogenic, and osteogenic progenitor stem cells as well as lineage-uncommitted pluripotent stem cells capable of forming muscle, adipocytes, cartilage, bone, fibroblasts, and endothelial cells. Flow cytometric analysis of adult stem cell populations revealed positive staining for CD34 and CD90 and negative staining for CD3, CD4, CD8, CD11c, CD33, CD36, CD38, CD45, CD117, Glycophorin-A, and HLA DR-II. Anat Rec 264:51-62,2001. (C) 2001 Wiley-Liss, Inc.