Pathogenicity and virulence of Candida dubliniensis: comparison with C. albicans.
Pathogenicity and virulence of Candida dubliniensis: comparison with C. albicans.
复制标题
都柏林念珠菌的致病性和毒力：与白色念珠菌的比较。
作者：
Maria Marluce dos Santos Vilela;Katsuhiko Kamei;Ayako Sano;Reiko Tanaka;Jun Uno;I. Takahashi;J. Ito;K. Yarita;M. Miyaji
都柏林念珠菌是一种新发现的真菌，经常从HIV阳性患者的口腔中分离出来。虽然对C. dubliniensis，很少有人知道这种酵母的致病生态。在这里，我们研究了与C有关的方面。dubliniensis与C.白色念珠菌当静脉注射到小鼠体内时，C. dubliniensis的成活率高于C.白色念珠菌。组织学分析表明，C. dubliniensis在感染器官中主要以酵母形式存在，而C.白色念珠菌转变为菌丝体。C.宿主的炎症反应是积极的。dubliniensis感染，轻度C.白色念珠菌感染酵母菌与人多形核白细胞共培养表明，C. dubliniensis比C.白色念珠菌C. Dubliniensis也更容易受到过氧化氢的毒性影响。体外培养时，C. dubliniensis比C. albicans，但芽管形成较快。当真菌在RPMI 1640中培养时，胎牛血清补充物抑制C. dubliniensis，但增强了C.白色念珠菌这些结果清楚地表明C. dubliniensis的毒力低于C.白色念珠菌
Candida dubliniensis is a newly described fungus that is frequently isolated from the oral cavities of HIV-positive patients. Although extensive studies have been performed on the phylogeny of C. dubliniensis, little is known about the pathogenic ecology of this yeast. Here we examined aspects related to C. dubliniensis in comparison with those of C. albicans. When injected intravenously into mice, C. dubliniensis had a higher survival rate than C. albicans. Histopathological analysis disclosed that C. dubliniensis remained mostly in the yeast form in the infected organs, whereas C. albicans changed into the mycelial form. The host inflammatory reaction was aggressive with C. dubliniensis infection and mild with C. albicans infection. Co-culture of the yeasts with human polymorphonuclear leukocytes disclosed that C. dubliniensis is more vulnerable to the fungicidal activity of leukocytes than C. albicans. C. dubliniensis was also more susceptible to the toxic effect of hydrogen peroxide. When cultured in vitro, C. dubliniensis grew more slowly than C. albicans, but the formation of germ tubes was faster. When the fungi were cultured in RPMI 1640, a fetal bovine serum supplement suppressed the growth of C. dubliniensis but enhanced that of C. albicans. These results clearly indicated that C. dubliniensis is less virulence than C. albicans.