The structure of intramolecular triplex DNA: atomic force microscopy study.
The structure of intramolecular triplex DNA: atomic force microscopy study.
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分子内三链 DNA 的结构：原子力显微镜研究。
DOI：
10.1006/jmbi.2001.5174
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Lyubchenko,YL
中科院分区：
文献类型：
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作者：
TinerSr,WJ;Potaman,VN;Sinden,RR;Lyubchenko,YL
我们应用原子力显微镜 (AFM) 对由镜像重复的嘌呤-嘧啶重复序列形成并通过负 DNA 超螺旋稳定的分子内三链体 (H-DNA) 进行直接成像。 H-DNA 在原子力显微镜图像中显示为厚度与 DNA 双链体不同的清晰突起。与现有模型一致，H-DNA 的形成导致双螺旋路径扭结。扭结形成锐角，使得侧翼 DNA 区域非常接近。与十字形和三向连接相比，侧翼 DNA 臂的活动性受到限制。 H-DNA 的结构特性对于启动子-增强子相互作用和其他 DNA 交易可能很重要。
We applied atomic force microscopy (AFM) for direct imaging of intramolecular triplexes (H-DNA) formed by mirror-repeated purine-pyrimidine repeats and stabilized by negative DNA supercoiling. H-DNA appears in atomic force microscopy images as a clear protrusion with a different thickness than DNA duplex. Consistent with the existing models, H-DNA formation results in a kink in the double helix path. The kink forms an acute angle so that the flanking DNA regions are brought in close proximity. The mobility of flanking DNA arms is limited compared with that for cruciforms and three-way junctions. Structural properties of H-DNA may be important for promoter-enhancer interactions and other DNA transactions.