Quartz-Seq : a highly reproducible and sensitive single-cell RNA-Seq reveals non-genetic gene expression heterogeneity
Quartz-Seq : a highly reproducible and sensitive single-cell RNA-Seq reveals non-genetic gene expression heterogeneity
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Quartz-Seq：高度可重复且灵敏的单细胞 RNA-Seq 揭示非遗传基因表达异质性
DOI：
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
H. Ueda
中科院分区：
文献类型：
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作者：
Yohei Sasagawa;I. Nikaido;Tetsutaro Hayashi;Hiroki Danno;Kenichiro D. Uno;Takeshi Imai;H. Ueda
高重复性和高灵敏度的单细胞RNA-SEQ方法将有助于理解非遗传细胞异质性的生物学作用和潜在机制。在这项研究中，我们报道了一种新的单细胞RNA-Seq方法，称为Quartz-Seq，与以前开发的方法相比，该方法具有更简单的操作流程、更高的重复性和灵敏度。我们证明了单细胞Quartz-Seq可以定量检测各种非遗传细胞异质性。该方法检测单细胞类型的不同细胞类型和不同的细胞周期时相。此外，该方法可以全面揭示同一细胞类型的单个细胞在相同的细胞周期时相之间的基因表达异质性。
A highly reproducible and sensitive single-cell RNA-Seq method will facilitate the understanding of the biological roles and underlying mechanisms of the non-genetic cellular heterogeneity. In this study, we report a novel single-cell RNA-Seq method called Quartz-Seq that has a simpler protocol and higher reproducibility and sensitivity compared to previously developed methods. We demonstrate that single-cell Quartz-Seq quantitatively detects various kind of non-genetic cellular heterogeneity. The method detects different cell types and different cell-cycle phases of a single-cell type. Moreover, this method can comprehensively reveal gene expression heterogeneity between single-cells of the same cell type at the same cell-cycle phase.
影响因子：
16
作者：
Bumgarner, Stacie L.;Neuert, Gregor;Voight, Benjamin F.;Symbor-Nagrabska, Anna;Grisafi, Paula;van Oudenaarden, Alexander;Fink, Gerald R.
通讯作者：
Fink, Gerald R.