Over-expression of Chinese cabbage phytocystatin 1 retards seed germination in Arabidopsis
Over-expression of Chinese cabbage phytocystatin 1 retards seed germination in Arabidopsis
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DOI：
10.1016/j.plantsci.2006.11.005
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发表时间：
2007-03-01
期刊：
影响因子：
5.2
通讯作者：
Lim, Chae Oh
中科院分区：
文献类型：
--
作者：
Hong, Joon Ki;Hwang, Jung Eun;Lim, Chae Oh
植物半胱氨酸蛋白酶抑制剂是植物半胱氨酸肽酶抑制剂，其参与蛋白质周转、程序性细胞死亡的内源性调节以及对昆虫和病原体的防御机制。从大白菜（B. Rapa L. SSP. pekinensis）。在萌发的种子中，种子萌发调节植物激素GA（4+7）和阿坝的处理分别导致BrCYS1表达的快速下降和轻微下降。在拟南芥中，组成型过表达的BrCYS1延缓发芽和幼苗生长，而这些都增强了抑制BrCYS1。此外，过度表达的BrCYS1引起的整体植物生长和发育的一般延迟，相反的效果，观察到在转基因植物中，BrCYS1的表达被抑制。此外，在转基因拟南芥中，BrCYS1抑制了储存的半胱氨酸肽酶活性。基于这些数据，我们认为，BrCYS1参与控制种子萌发，通过调节存储半胱氨酸肽酶活性。(c)2006爱思唯尔爱尔兰有限公司保留所有权利。
Phytocystatins are plant cysteine peptidase inhibitors that are implicated in the endogenous regulation of protein turnover, programmed cell death, and in defense mechanisms against insects and pathogens. cDNA encoding the phytocystatin BrCYS1 (Brassica rapa phytocystatin 1) has been isolated from the flower buds of Chinese cabbage (B. rapa L. ssp. pekinensis). In germinating seeds, treatment with the seed germination regulatory phytohormones GA(4+7) and ABA resulted in a rapid decrease and a slight reduction in BrCYS1 expression, respectively. In Arabidopsis, constitutive over-expression of BrCYS1 retarded germination and seedling growth, whereas these were enhanced by suppression of BrCYs1. In addition, over-expression of BrCYS1 caused a general retardation of overall plant growth and development; the opposite effect was observed in transgenic plants in which BrCYS1 expression was suppressed. Additionally, stored cysteine peptidase activity was inhibited by BrCYS1 in transgenic Arabidopsis. Based on these data, we suggest that BrCYS1 participates in the control of seed germination by regulating stored cysteine peptidase activity. (c) 2006 Elsevier Ireland Ltd. All rights reserved.