Angiotensinogen gene expression in adipose tissue: Analysis of obese models and hormonal and nutritional control
Angiotensinogen gene expression in adipose tissue: Analysis of obese models and hormonal and nutritional control
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DOI：
10.1152/ajpregu.1997.273.1.r236
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发表时间：
1997-07-01
影响因子：
2.8
通讯作者：
Moustaid, N
中科院分区：
文献类型：
--
作者：
Jones, BH;Standridge, MK;Moustaid, N
血管紧张素II（ANC; II）的合成最近已被描述在脂肪细胞中，并已与肥胖的调节。血管紧张素原（AGT），血管紧张素II形成的底物，先前显示在肥胖（ob/ob和db/db）小鼠的脂肪组织中升高，并通过营养操作进行调节。然而，目前尚不清楚脂肪AGT的过度表达是否可以扩展到其他肥胖模型，以及激素和/或营养因素是否直接调节脂肪细胞中AGT的表达。我们通过分析肥胖Zucker大鼠、Viable yellow（A（vy））小鼠和人类脂肪组织中AGT mRNA水平，以及用胰岛素、葡萄糖和β-肾上腺素能激动剂处理3T3-L1脂肪细胞，研究了这些可能性。我们证明，AGT mRNA减少了类似的50和80%，分别在脂肪组织的肥胖与瘦Zucker大鼠和A（vy）小鼠。我们还报告，AGT在人类脂肪组织中以不同的水平表达。最后，我们表明，AGT mRNA的上调胰岛素和下调β-肾上腺素能刺激脂肪细胞。
Synthesis of angiotensin Ii (ANC; II) has recently been described in adipose cells and has been linked to regulation of adiposity. Angiotensinogen (AGT), the substrate from which ANG II is formed, was previously shown to be elevated in adipose tissue of obese (ob/ob and db/db) mice and regulated by nutritional manipulation. It is unknown, however, whether overexpression of adipose AGT can be extended to other models of obesity and whether hormonal and/or nutritional factors directly regulate AGT expression in adipocytes, We investigated these possibilities by analyzing AGT mRNA levels in adipose tissue of obese Zucker rats, Viable yellow (A(vy)) mice, and humans and by treating 3T3-L1 adipocytes with insulin, glucose, and a beta-adrenergic agonist. We demonstrate that AGT mRNA is decreased by similar to 50 and 80%, respectively, in adipose tissue of obese vs. lean Zucker rats and A(vy) mice. We also report that AGT is expressed at variable levels in human adipose tissue. Finally, we show that AGT mRNA is upregulated by insulin and downregulated by beta-adrenergic stimulation in adipocytes.