Micro-C XL: assaying chromosome conformation from the nucleosome to the entire genome
Micro-C XL: assaying chromosome conformation from the nucleosome to the entire genome
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DOI：
10.1038/nmeth.4025
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发表时间：
2016-12-01
期刊：
影响因子：
48
通讯作者：
Rando, Oliver J.
中科院分区：
文献类型：
--
作者：
Hsieh, Tsung-Han S.;Fudenberg, Geoffrey;Rando, Oliver J.
我们提出了一种改进的方法Micro-C XL，用于分析染色体的折叠在montesome分辨率。使用长交联剂和分离不溶性染色质，Micro-C XL提高信噪比。微-C XL图的芽殖和裂殖酵母基因组捕获短距离染色体纤维的功能，如染色体相互作用域和更高阶的功能，如着丝粒聚类。Micro-C XL提供了一个单一的分析，以询问从核小体到全基因组的长度尺度上的染色体折叠。
We present Micro-C XL, an improved method for analysis of chromosome folding at mononucleosome resolution. Using long crosslinkers and isolation of insoluble chromatin, Micro-C XL increases signal-to-noise ratio. Micro-C XL maps of budding and fission yeast genomes capture both short-range chromosome fiber features such as chromosomally interacting domains and higher order features such as centromere clustering. Micro-C XL provides a single assay to interrogate chromosome folding at length scales from the nucleosome to the full genome.