Desulfoluna limicola sp. nov., a sulfate-reducing bacterium isolated from sediment of a brackish lake
Desulfoluna limicola sp. nov., a sulfate-reducing bacterium isolated from sediment of a brackish lake
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DOI：
10.1007/s00203-022-03259-0
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发表时间：
2022-09
影响因子：
2.8
通讯作者：
Miho Watanabe;Ayaka Takahashi;Hisaya Kojima;M. Fukui
中科院分区：
文献类型：
--
作者：
Miho Watanabe;Ayaka Takahashi;Hisaya Kojima;M. Fukui
从日本某咸水湖沉积物中分离到一株硫酸盐还原菌ASN 36 T。ASN 36 T菌株细胞无运动能力，呈杆状，长2.0-4.9 μm，宽0.6-0.9 μm。在5-35 °C下观察到生长，最佳生长温度为25-30 °C。生长的pH范围为6.6-8.8，最适pH为7.3。主要脂肪酸为C16：1ω 7 c和C16：0。在硫酸盐还原条件下，菌株ASN 36 T利用乳酸、苹果酸、丙酮酸、丁酸、乙醇、丁醇、甘油、酵母膏和H2/CO2作为生长底物。发酵生长发生在苹果酸和丙酮酸上。新的分离物使用硫酸盐、亚硫酸盐和硫代硫酸盐作为电子受体。菌株ASN 36 T的染色体长度为6.3Mbp，G + C含量为55.1mol%。16 S rRNA基因分析表明菌株ASN 36 T与脱硫醇属的种有关。总体基因组相关性指数表明菌株ASN 36 T不属于任何现存物种。与最接近的亲戚相比，菌株ASN 36 T缺乏有机卤化物呼吸所需的还原脱卤酶基因，并且不使用卤代芳烃作为电子受体。根据菌株ASN 36 T（= DSM 111985 T = JCM 39257 T）的基因组和表型特征，提出菌株ASN 36 T为一新种--Desulfoluna limicolasp的模式菌株。Nov.
A novel sulfate-reducing bacterium, strain ASN36T, was isolated from sediment of a brackish lake in Japan. Cells of strain ASN36Twere not motile and rod-shaped, with length of 2.0–4.9 μm and width of 0.6–0.9 μm. Growth was observed at 5–35 °C with an optimum growth temperature of 25–30 °C. The pH range for growth was 6.6–8.8 with an optimum pH of 7.3. Major fatty acids were C16:1ω7cand C16:0. Under sulfate-reducing conditions, strain ASN36Tutilized lactate, malate, pyruvate, butyrate, ethanol, butanol, glycerol, yeast extract and H2/CO2as growth substrate. Fermentative growth occurred on malate and pyruvate. The novel isolate used sulfate, sulfite and thiosulfate as electron acceptors. The genome of strain ASN36Tis composed of a chromosome with length of 6.3 Mbp and G + C content of 55.1 mol%. Analyses of the 16S rRNA gene indicated that strain ASN36Tis related toDesulfolunaspecies. Overall genome relatedness indices indicated that strain ASN36Tdoes not belong to any existing species. In contrast to the closest relatives, strain ASN36Tlacks genes for reductive dehalogenase required for organohalide respiration and does not use halogenated aromatics as electron acceptors. On the basis of its genomic and phenotypic properties, strain ASN36T(= DSM 111985T= JCM 39257T) is proposed as the type strain of a new species,Desulfoluna limicolasp. nov.