Transcriptome meets metabolome: hierarchical and metabolic regulation of the glycolytic pathway
Transcriptome meets metabolome: hierarchical and metabolic regulation of the glycolytic pathway
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DOI：
10.1016/s0014-5793(01)02613-8
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发表时间：
2001-07-06
期刊：
影响因子：
3.5
通讯作者：
Westerhoff, HV
中科院分区：
文献类型：
--
作者：
ter Kuile, BH;Westerhoff, HV
信息从 DNA 流向 RNA 再流向蛋白质以发挥功能的事实表明，调控是“分层的”，即以基因表达的调控为主。然而，在代谢水平占主导地位的情况下，mRNA水平和功能之间不存在定量关系。我们在这里开发了一种方法来量化代谢和层次调节的相对贡献。将这种方法应用于三种寄生原生生物的糖酵解通量，我们得出结论，它很少仅受基因表达的调节。这使人们对转录组和蛋白质组分析是否足以评估生物学功能产生强烈怀疑。 (C) 2001 年欧洲生化学会联合会，由 Elsevier Science B.V 出版。保留所有权利。
The fact that information flows from DNA to RNA to protein to function suggests that regulation is 'hierarchical', i.e. dominated by regulation of gene expression. In the case of dominant regulation at the metabolic level, however, there is no quantitative relationship between mRNA levels and function. we here develop a method to quantitate the relative contributions of metabolic and hierarchical regulation. Applying this method to the glycolytic flux in three species of parasitic protists, we conclude that it is rarely regulated by gene expression alone. This casts strong doubts on whether transcriptome and proteome analysis suffices to assess biological function. (C) 2001 Federation of European Biochemical Societies, Published by Elsevier Science B.V. All rights reserved.