Molecular characterization of novel P[14],G8 bovine group A rotavirus, Sun9, isolated in Japan
Molecular characterization of novel P[14],G8 bovine group A rotavirus, Sun9, isolated in Japan
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DOI：
10.1016/j.virusres.2004.04.016
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发表时间：
2004-09-15
期刊：
影响因子：
5
通讯作者：
Sato, M
中科院分区：
文献类型：
--
作者：
Fukai, K;Saito, T;Sato, M
本研究从日本栃木县犊牛腹泻中分离出一种新型牛a群轮状病毒（RV-A） Sun9，通过编码VP4和VP7的基因组片段的序列分析对其进行了遗传鉴定。Sun9的VP4和VP7基因组片段的核苷酸序列和推导出的氨基酸序列与P[14]人、羊RV-As（80.2 ~ 88.7%和90.9 ~ 94.8%）和G8牛、人RV-As（83.1 ~ 95.5%和92.3 ~ 98.2%）具有高度同源性。对编码VP4和VP7的基因组片段的核苷酸序列进行系统发育分析，将Sun9分类为P[141]和G8。虽然以前的报道表明，迄今为止分离的p[14]，G8人类RV-A是从人与牛的RV-A重组中获得的，或者是从牛的RV-A向人的种间传播中获得的，但尚未有p[14]，G8牛RV-A的报道。太阳9可能是上述假设的初步直接证据。(C) 2004 Elsevier B.V.版权所有
In this study, a novel bovine group A rotavirus (RV-A), Sun9, isolated from calf diarrhea in the Tochigi Prefecture, Japan, was characterized genetically by the sequence analysis of the genome segments encoding VP4 and VP7. The nucleotide and deduced amino acid sequences of the genome segments encoding VP4 and VP7 of Sun9 revealed high homology with P[14] human and lapine RV-As (80.2-88.7% and 90.9-94.8%) and G8 bovine and human RV-As (83.1-95.5% and 92.3-98.2%). Sun9 was also classified into P[141 and G8 in the phylogenetic analysis of the nucleotide sequences of the genome segments encoding VP4 and VP7. Although previous reports have suggested that P[14],G8 human RV-As isolated until now were obtained from the reassortment between human and bovine RV-As, or the interspecies transmission of bovine RV-A to human, no P[14],G8 bovine RV-A has yet been reported. Sun9 may be initial direct evidence of the above hypothesis. (C) 2004 Elsevier B.V. All rights reserved.