THE GENE FOR HUMAN P53 CELLULAR TUMOR-ANTIGEN IS LOCATED ON CHROMOSOME-17 SHORT ARM (17P13)
THE GENE FOR HUMAN P53 CELLULAR TUMOR-ANTIGEN IS LOCATED ON CHROMOSOME-17 SHORT ARM (17P13)
复制标题
DOI：
10.1073/pnas.83.1.130
复制
发表时间：
1986-01-01
影响因子：
11.1
通讯作者：
GIVOL, D
中科院分区：
文献类型：
--
作者：
MCBRIDE, OW;MERRY, D;GIVOL, D
从猴病毒40转化的人成纤维细胞的cdna文库中分离到一个与小鼠p53探针交叉杂交的克隆。这一克隆的人P53基因被用作探针，用于检测从分离了人类染色体的人-啮齿动物体细胞杂交中获得的DNA。结果表明，人P53基因位于17号染色体上。此外，对含有17号染色体易位的人细胞制备的杂交种进行Southern分析，可以将人P53基因定位到该染色体短臂的最远端(17p13)。用人P53探针与人的中期分裂相进行原位杂交，证实了P53基因定位于17p13。
A clone that cross-hybridizes with a mouse p53 probe has been isolated from a cDNA library of simian virus 40-transformed human fibroblasts. This cloned human p53 cDNA was used as a probe to examine DNAs obtained from human-rodent somatic cell hybrids that have segregated human chromosomes. The results show that the human p53 gene is located on chromosome 17. In addition, Southern analysis of hybrids prepared from human cells containing a chromosome 17 translocation allowed regional localization of the human p53 gene to the most distal band on the short arm of this chromosome (17p13). Localization of the p53 gene to 17p13 was confirmed by in situ hybridization of metaphase spreads with the human p53 probe.