Predicting Species-Resolved Macronutrient Acquisition during Succession in a Model Phototrophic Biofilm Using an Integrated 'Omics Approach.
Predicting Species-Resolved Macronutrient Acquisition during Succession in a Model Phototrophic Biofilm Using an Integrated 'Omics Approach.
复制标题
DOI：
10.3389/fmicb.2017.01020
复制
发表时间：
2017
影响因子：
5.2
通讯作者：
Romine MF
中科院分区：
文献类型：
--
作者：
Lindemann SR;Mobberley JM;Cole JK;Markillie LM;Taylor RC;Huang E;Chrisler WB;Wiley HS;Lipton MS;Nelson WC;Fredrickson JK;Romine MF
人们对微生物群落中养分获取和种间交换的原则以及这些交换如何影响群落生产力知之甚少。在这里，我们研究了单蓝藻菌群中能量和常量营养素的获取，该菌群的所有成员都存在物种解析的基因组信息，使我们能够使用多组学方法来预测物种获取资源的能力并检查演替过程中资源获取基因的表达。代谢重建表明，大多数异养群落成员缺乏直接获取培养基中提供的无机营养物所需的基因，表明代谢高度相互依赖性。 UCC-O 联盟中唯一的初级生产者是蓝细菌席藻 sp。 OSCR 显示能量收集、碳固定以及硝酸盐和硫酸盐还原蛋白的表达下降，但磷酸盐转运蛋白的表达在 28 天内急剧增加。大多数异养成员在演替过程中同样表现出磷饥饿的迹象。尽管异养生物对磷限制的反应相似，但它们表现出氮获取基因的物种特异性表达。这些结果表明，当生物体直接竞争有限的磷酸盐时，围绕氮源的生态位划分可能会构建群落。氮源周围的这种生态位互补性可能会增加磷酸盐限制的光养群落的群落多样性和生产力。
The principles governing acquisition and interspecies exchange of nutrients in microbial communities and how those exchanges impact community productivity are poorly understood. Here, we examine energy and macronutrient acquisition in unicyanobacterial consortia for which species-resolved genome information exists for all members, allowing us to use multi-omic approaches to predict species’ abilities to acquire resources and examine expression of resource-acquisition genes during succession. Metabolic reconstruction indicated that a majority of heterotrophic community members lacked the genes required to directly acquire the inorganic nutrients provided in culture medium, suggesting high metabolic interdependency. The sole primary producer in consortium UCC-O, cyanobacterium Phormidium sp. OSCR, displayed declining expression of energy harvest, carbon fixation, and nitrate and sulfate reduction proteins but sharply increasing phosphate transporter expression over 28 days. Most heterotrophic members likewise exhibited signs of phosphorus starvation during succession. Though similar in their responses to phosphorus limitation, heterotrophs displayed species-specific expression of nitrogen acquisition genes. These results suggest niche partitioning around nitrogen sources may structure the community when organisms directly compete for limited phosphate. Such niche complementarity around nitrogen sources may increase community diversity and productivity in phosphate-limited phototrophic communities.