The T-cell receptor repertoire is strikingly similar in older nude mice compared to normal adult mice.
The T-cell receptor repertoire is strikingly similar in older nude mice compared to normal adult mice.
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与正常成年小鼠相比，老年裸鼠的 T 细胞受体库惊人地相似。
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
T. Mak
中科院分区：
文献类型：
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作者：
D. Ferrick;S. Sambhara;B. Chadwick;R. Miller;T. Mak
由于没有正常的胸腺，裸鼠的特征是成熟T细胞数量少。在年老的裸鼠中，可以发现相当数量的Thy-1+、CD 8 + T细胞，并且在小得多的程度上，可以发现CD 4 + T细胞。最近的数据表明，某些γ和δ链可变区（V）、多样性区（D）、连接区（J）和恒定区（C）基因与正常小鼠中γ δ-T细胞库的时间和空间组织相关。在这项研究中，我们观察到主要使用V γ 2 J γ 1C γ 1和V δ 5 J δ 1C δ在脾脏中的老年裸鼠。在皮肤中，观察到V γ 3 J γ 1C γ 1和V δ 1 J δ 2C δ基因片段的近排他性表达。此外，从裸鼠皮肤分离树突状表皮细胞（DEC）克隆，并表达由V γ 3 J γ 1C γ 1和V δ 1 J δ 2C δ组成的γ δ受体。初步结果显示，该克隆仅杀死YAC（和NK靶标），而不杀死其他肿瘤靶标。抗CD 3单克隆抗体可使杀伤重定向，表明该受体具有潜在的功能。这些结果表明，在没有正常胸腺发育的情况下，裸鼠不仅产生具有裂解潜力的γ δ-T细胞，而且还设法表达在空间排列上与正常小鼠相似的γ δ-TCR。这表明γ δ-TCR个体发育的要求可能存在差异。
Due to the absence of a normal thymus, nude mice are characterized by low numbers of mature T cells. It has been shown in older nude mice that appreciable numbers of Thy-1+, CD8+ and, to a much lesser extent, CD4+ T cells can be found. A second T-cell receptor (TCR), gamma delta-TCR, has been shown to be expressed by a large number of T cells in young and old nude mice. Recent data suggest that certain gamma and delta chain variable (V), diversity (D), joining (J) and constant (C) region genes are associated with both temporal and spatial organization of the gamma delta-T-cell repertoire in normal mice. In this study we observed the predominant use of V gamma 2J gamma 1C gamma 1 and V delta 5J delta 1C delta in the spleen of older nude mice. In the skin, a near exclusive expression of V gamma 3J gamma 1C gamma 1 and V delta 1J delta 2C delta gene segments was observed. In addition, a dendritic epidermal cell (DEC) clone was isolated from the skin of a nude mouse and expressed a gamma delta receptor consisting of V gamma 3J gamma 1C gamma 1 and V delta 1J delta 2C delta. Preliminary results show that this clone kills only YAC (and NK target) and not other tumor targets. The killing can be redirected by anti-CD3 monoclonal antibody which suggests that the receptor is potentially functional. These results demonstrate that without normal thymus development nude mice not only produce gamma delta-T cells with a lytic potential, but also manage to express gamma delta-TCR's that are similar in spatial arrangement to normal mice. This suggests a possible difference in the requirements for gamma delta-TCR ontogeny.