Water-Soluble Nile Blue Derivatives: Syntheses and Photophysical Properties
Water-Soluble Nile Blue Derivatives: Syntheses and Photophysical Properties
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DOI：
10.1002/chem.200801104
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发表时间：
2009-01-01
影响因子：
4.3
通讯作者：
Burgess, Kevin
中科院分区：
文献类型：
--
作者：
Jose, Jiney;Ueno, Yuichiro;Burgess, Kevin
通过在相对温和的条件（90 ℃，N，N-二甲基甲酰胺，不添加酸）下进行的缩合反应制备四种水溶性2-羟基-尼罗蓝衍生物1a、1b、2a和2b。这些荧光探针具有比两种已知的水溶性尼罗蓝衍生物更有利的荧光特性。具体而言，它们在水性介质中的量子产率和荧光发射的锐度方面上级已知染料。浓度依赖性的紫外吸收和荧光发射研究表明，染料不聚集在水溶液中的浓度小于1-4 μ m。现在的水溶性材料1a、1b、2a和2b在荧光光谱的期望区域（λ =670- 675 nm）中发射。总的来说，它们对于在水性环境中标记生物分子具有潜在的意义。
Four water-soluble 2-hydroxy-Nile Blue derivatives, 1a, 1b, 2 a, and 2b, were prepared by condensation reactions performed under relatively mild conditions (90 degrees C, N,N-diniethylformamide with no added acid). These fluorescent probes had more favorable fluorescence characteristics than two known water-soluble Nile Blue derivatives. Specifically, they were superior to the known dyes with respect to their quantum yields in aqueous media and the sharpness of their fluorescence emissions. Concentration-dependant UV absorption and fluorescence emission studies indicated that the dyes did not aggregate in aqueous solution at concentrations of less than 1-4 mu m. The now water-soluble materials 1a, 1b, 2a, and 2b emit in a desirable region of the fluorescence spectrum (lambda=670-675nm). Overall they are potentially interesting for labeling biomolecules in aqueous environments.